PCE3 Plays a Role in the Reproduction of Male Nilaparvata lugens.
PCE3 Plays a Role in the Reproduction of Male Nilaparvata lugens.
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PCE3 在雄性褐飞虱的繁殖中发挥作用。
DOI：
10.3390/insects12020114
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发表时间：
2021-01-28
期刊：
影响因子：
3
通讯作者：
Xu Y
中科院分区：
文献类型：
--
作者：
Zheng RE;Ji J;Wu J;Zhang R;Li Y;Yu X;Xu Y
褐飞虱（Nilaparvata lugens）是危害水稻的主要害虫之一。利用RNAi技术是控制该害虫的一种可行策略。在这项研究中，我们评估了PCE 3在男性BPH的发展和生殖中的重要性。结果表明，PCE 3基因可以调控雄性内生殖器的发育，并降低与靶向N.褐飞虱PCE 3基因，导致卵不能孵化。我们的研究结果表明，PCE 3是一个重要的基因在调节男性生殖力和一个有希望的目标，控制BPH。褐飞虱促凝酶（Nilaparvatalugens proclotting enzymes，NlPCE）属于夹域丝氨酸蛋白酶（clip-SP）家族，是节肢动物特有的蛋白酶家族。NlPCE 3在雌性黑腹蛛中调节卵的产生和发育。lugens，但在雄性N.卢夫斯不清楚。在本研究中，qPCR分析显示NlPCE 3在三种不同的组织（肠、睾丸和脂肪体）中表达。RNAi显示，dsNlPCE 3注射使雄性输精管变薄，并降低了与dsNlPCE 3处理的雄性交配的雌性的产卵水平，导致卵不能孵化。免疫荧光染色显示NlPCE 3在雄性内生殖器中广泛表达。然而，注射dsNlPCE 3后，NlPCE 3的表达在雄性内生殖器中弥漫，其外周细胞似乎降解。总之，这些结果表明，NlPCE 3是重要的生殖在男性N。lugens。
The brown planthopper (BPH), Nilaparvata lugens, is one of the most harmful rice crop pest insects. The use of RNAi is a feasible strategy for controlling this pest. In this study, we evaluated the importance of PCE3 in the development and reproduction of male BPH. We found that PCE3 could regulate the development of the male internal genitalia and reduce the oviposition level of the females that mated with males treated with dsRNA targeting the N. lugens PCE3 gene, causing eggs not to hatch. Our findings indicate that PCE3 is an important gene in regulating male fecundity and a promising target for controlling BPH. Nilaparvata lugens proclotting enzymes (NlPCEs) belong to the clip domain serine protease (clip-SP) family, which is a characteristic protease family in arthropods. NlPCE3 was previously reported to regulate egg production and development in female N. lugens, but its role in male N. lugens is unclear. In the present study, qPCR analysis showed that NlPCE3 was expressed in three different tissues (gut, testis and fat body). RNAi revealed that dsNlPCE3 injection made the male vas deferens thinner and reduced the oviposition level of the females that mated with dsNlPCE3-treated males, causing eggs not to hatch. Furthermore, immunofluorescence staining showed that NlPCE3 was widely expressed in the male internal genitalia. However, after dsNlPCE3 injection, expression of NlPCE3 was diffuse in the male internal genitalia, whose peripheral cells seemed degraded. Overall, these results indicate that NlPCE3 is important for reproduction in male N. lugens.
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