Diversity of bacteria and archaea in the deep-sea low-temperature hydrothermal sulfide chimney of the Northeastern Pacific Ocean
Diversity of bacteria and archaea in the deep-sea low-temperature hydrothermal sulfide chimney of the Northeastern Pacific Ocean
复制标题
东北太平洋深海低温热液硫化物烟囱细菌和古菌多样性
DOI：
10.5897/ajb11.2692
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发表时间：
2011-01
影响因子：
--
通讯作者：
Huai-Yang Zhou
中科院分区：
文献类型：
--
作者：
Xia Ding;Xiao-Jue Peng;Xiao-Tong Peng;Huai-Yang Zhou
在过去的十年里，我们对热液喷口微生物的多样性和作用的了解有了很大的扩展，但对低温热液硫化物烟囱中微生物的多样性却知之甚少。本研究采用变性梯度凝胶电泳（DGGE）和16S rDNA测序技术检测了东北太平洋深海低温热液硫化物烟囱从外部到内部的微生物丰度和多样性。 DGGE图谱显示，在烟囱壁的所有三个区域中都可以检测到细菌和古细菌，并且不同区域内的微生物群落的组成存在很大差异。总体而言，对于古细菌来说，烟囱壁最外层区域的细胞丰度最大。对于细菌来说，三个区域之间的细胞丰度没有显着差异。此外，系统发育分析显示，外部区优势菌门为 Verrucomicrobia 和 Deltaproteobacteria，中部区优势菌为 β Proteobacteria，内部区丰富微生物为 Bacillus。古菌方面，中外区以未分类古菌为主，内区以产甲烷菌为主。总而言之，这些结果表明，硫化物烟囱壁上的微生物群落组成发生了转变。我们的研究结果为了解深海低温热液硫化物烟囱中微生物的多样性和系统发育提供了有用的信息。
Our knowledge of the diversity and role of hydrothermal vents microorganisms has considerably expanded over the past decade, while little is known about the diversity of microorganisms in low-temperature hydrothermal sulfide chimney. In this study, denaturing gradient gel electrophoresis (DGGE) and 16S rDNA sequencing were used to examine the abundance and diversity of microorganisms from the exterior to the interior of the deep sea low-temperature hydrothermal sulfide chimney of the Northeastern Pacific Ocean. DGGE profiles revealed that both bacteria and archaea could be examined in all three zones of the chimney wall and the compositions of microbial communities within different zones were vastly different. Overall, for archaea, cell abundance was greatest in the outermost zone of the chimney wall. For bacteria, there was no significant difference in cell abundance among three zones. In addition, phylogenetic analysis revealed that Verrucomicrobia and Deltaproteobacteria were the predominant bacterial members in exterior zone, beta Proteobacteria were the dominant members in middle zone, and Bacillus were the abundant microorganisms in interior zone. As to archaea, the middle and exterior were dominated by unclassified archaea, while interior zone was dominated by methanogens. Taken together, these results show that there was a transition in the composition of microbial communities across the sulfide chimney wall. Our findings provide helpful information for understanding the diversity and phylogeny of microorganisms in the deep-sea low temperature hydrothermal sulfide chimney.
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影响因子：
4.2
作者：
V. Marteinsson;J. Birrien;C. Jeanthon;D. Prieur
通讯作者：
V. Marteinsson;J. Birrien;C. Jeanthon;D. Prieur
DOI：
10.1099/ijs.0.63430-0
发表时间：
2005-03
影响因子：
2.8
作者：
James W. Voordeckers;V. Starovoytov;C. Vetriani
通讯作者：
James W. Voordeckers;V. Starovoytov;C. Vetriani
影响因子：
4.4
作者：
MOYER, CL;DOBBS, FC;KARL, DM
通讯作者：
KARL, DM
影响因子：
4.4
作者：
Kato, Shingo;Takano, Yoshinori;Yamagishi, Akihiko
通讯作者：
Yamagishi, Akihiko
影响因子：
4.4
作者：
Schrenk, MO;Kelley, DS;Baross, JA
通讯作者：
Baross, JA