Detection of coronaviruses in bats of various species in Italy.
Detection of coronaviruses in bats of various species in Italy.
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DOI：
10.3390/v5112679
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发表时间：
2013-10-31
期刊：
影响因子：
--
通讯作者：
Boniotti MB
中科院分区：
文献类型：
--
作者：
Lelli D;Papetti A;Sabelli C;Rosti E;Moreno A;Boniotti MB
蝙蝠是许多哺乳动物冠状病毒的天然宿主，在人类中发现严重急性呼吸综合征（SARS）和中东呼吸综合征（MERS）CoV后，这些病毒重新受到关注。本研究描述了2010年至2012年在意大利蝙蝠中发现的冠状病毒和β冠状病毒的鉴定和分子特征。采用泛冠状病毒RT-PCR检测了69份粪便样本和126具尸体。在7份粪便样本和9具尸体中检测到冠状病毒RNA。RNA依赖性RNA聚合酶序列片段的系统发育分析有助于鉴定来自库尔伏翼（库氏伏翼）的两种β-冠状病毒，来自小蹄蝠（菊头蝠）的三种2b进化枝β-冠状病毒，以及来自库尔伏翼、普通伏翼（Nyctalus sputula）和萨维伏翼（Hypsugo savii）的10种2c进化枝β-冠状病毒。这项研究填补了意大利蝙蝠冠状病毒生态学知识的实质性空白，并扩展了目前对进化枝2c β冠状病毒的知识，并从蝙蝠中获得了以前未被描述为这些病毒宿主的新序列。
Bats are natural reservoirs for many mammalian coronaviruses, which have received renewed interest after the discovery of the severe acute respiratory syndrome (SARS) and the Middle East respiratory syndrome (MERS) CoV in humans. This study describes the identification and molecular characterization of alphacoronaviruses and betacoronaviruses in bats in Italy, from 2010 to 2012. Sixty-nine faecal samples and 126 carcasses were tested using pan-coronavirus RT-PCR. Coronavirus RNAs were detected in seven faecal samples and nine carcasses. A phylogenetic analysis of RNA-dependent RNA polymerase sequence fragments aided in identifying two alphacoronaviruses from Kuhl’s pipistrelle (Pipistrellus kuhlii), three clade 2b betacoronaviruses from lesser horseshoe bats (Rhinolophus hipposideros), and 10 clade 2c betacoronaviruses from Kuhl’s pipistrelle, common noctule (Nyctalus noctula), and Savi’s pipistrelle (Hypsugo savii). This study fills a substantive gap in the knowledge on bat-CoV ecology in Italy, and extends the current knowledge on clade 2c betacoronaviruses with new sequences obtained from bats that have not been previously described as hosts of these viruses.
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DOI：
10.1016/j.jinf.2012.10.002
发表时间：
2012-12
期刊：
The Journal of infection
影响因子：
--
作者：
Chan JF;Li KS;To KK;Cheng VC;Chen H;Yuen KY
通讯作者：
Yuen KY
德国北部蝙蝠中I组冠状病毒的检测和患病率模式。
影响因子：
11.8
作者：
Gloza-Rausch, Florian;Ipsen, Anne;Seebens, Antje;Goettsche, Matthias;Panning, Marcus;Drexler, Jan Felix;Petersen, Nadine;Annan, Augustina;Grywna, Klaus;Mueller, Marcel;Pfefferle, Susanne;Drosten, Christian
通讯作者：
Drosten, Christian
影响因子：
3.3
作者：
Decaro N;Buonavoglia C
通讯作者：
Buonavoglia C
影响因子：
11.8
作者：
Carrington, Christine V. F.;Foster, Jerome E.;Zhu, Hua Chen;Zhang, Jin Xia;Smith, Gavin J. D.;Thompson, Nadin;Auguste, Albert J.;Ramkissoon, Vernie;Adesiyun, Abiodun A.;Guan, Yi
通讯作者：
Guan, Yi
影响因子：
11.8
作者：
Pfefferle S;Oppong S;Drexler JF;Gloza-Rausch F;Ipsen A;Seebens A;Müller MA;Annan A;Vallo P;Adu-Sarkodie Y;Kruppa TF;Drosten C
通讯作者：
Drosten C