Heterotypic cell pair co-culturing on patterned microarrays.
Heterotypic cell pair co-culturing on patterned microarrays.
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DOI：
10.1039/c2lc40349h
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发表时间：
2012-09-07
期刊：
影响因子：
6.1
通讯作者：
Reich DH
中科院分区：
文献类型：
--
作者：
Felton EJ;Copeland CR;Chen CS;Reich DH
我们提出了一种成对共培养技术，该技术在图案化阵列中产生大量的异型细胞对。光刻图案化产生具有数千个陷阱的阵列，每个陷阱设计为仅容纳两个细胞并将它们限制在这些位点进行共培养。引入了两种变体：一种是将两种细胞类型的混合物沉积到阵列上的随机接种方法，另一种是将铁磁薄膜结合到阵列中并通过偶极相互作用将已附着到铁磁纳米线上的细胞吸引到阵列位置的方法。阵列技术包括利用定制的图像分析软件，从多通道荧光图像中提取数据，并记录每个陷阱中细胞的信息，从而获得准确的高统计数据。该技术的适用性，证明了在实验中检查增殖率对牛肺动脉内皮细胞和平滑肌细胞。结果表明，异型相互作用有利于平滑肌细胞增殖，而不利于内皮细胞增殖。这是可以用这种方法探测的各种细胞-细胞相互作用的一个例子。
We present a pair-wise co-culturing technique that creates large numbers of heterotypic cell pairs in patterned arrays. Lithographic patterning produces arrays with thousands of traps, each designed to accommodate only two cells and confine them at these sites for co-culturing. Two variants are introduced: a random seeding method that sediments a mixture of two cell types onto the array, and an approach that incorporates ferromagnetic thin films into the arrays and attracts cells that have been attached to ferromagnetic nanowires to the array sites through dipole interactions. The array technique includes the utilization of custom image analysis software that extracts data from multi-channel fluorescence images and records information about the cells in every trap, enabling the acquisition of accurate, high-statistics data. The applicability of the technique was demonstrated in experiments examining proliferation rates in pairs of bovine pulmonary artery endothelial and smooth muscle cells. Results demonstrated that heterotypic interactions favored smooth muscle cell proliferation while disfavoring endothelial cell proliferation. This is one example of a variety of cell-cell interactions that could be probed with this method.
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影响因子：
64.5
作者：
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通讯作者：
Sheetz, MP
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作者：
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作者：
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影响因子：
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作者：
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