Morphology and connectivity of the small bistratified A8 amacrine cell in the mouse retina.
Morphology and connectivity of the small bistratified A8 amacrine cell in the mouse retina.
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DOI：
10.1002/cne.23752
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发表时间：
2015-07-01
影响因子：
2.5
通讯作者：
Haverkamp, Silke
中科院分区：
文献类型：
--
作者：
Lee, Sammy C. S.;Meyer, Arndt;Schubert, Timm;Hueser, Laura;Dedek, Karin;Haverkamp, Silke
关键词：
无长突细胞在哺乳动物视网膜中包括约30种形态类型。最近研究了一些GABA能宽视野无长突细胞的突触连接和功能，然而，除了视杆通路特异性AII无长突细胞外，还没有研究小鼠视网膜中甘氨酸能小视野无长突细胞的连接。在这里，我们研究了小视野A8无长突细胞的形态和连接模式。小鼠视网膜A_8细胞为双层细胞，在内网层的ON亚层中有小叶突起，在OFF亚层中有树状树突。在出生后第8天首次可见明显的双层形态，在P13时，在眼睛张开周围达到成人形状。A8细胞与双极细胞和神经节细胞的连接性通过使用各种细胞标记物与突触标记物组合的双重和三重免疫标记实验来研究。我们的数据表明，A8无长突细胞接受来自OFF和ON锥体双极细胞的突触能输入。此外，A8细胞通过间隙连接与ON锥体双极细胞偶联，并通过甘氨酸受体（GlyR）亚单位α1向OFF锥体双极细胞和ON A型神经节细胞提供抑制性输入。自发性甘氨酸能突触后电流和GlyR免疫标记的测量显示，A8细胞表达含有α2亚基的GlyR。两者合计，bisteratified A8细胞与视杆通路特异性AII无长突细胞的锥双极细胞的突触接触非常相似。然而，与AII细胞不同，A8无长突细胞向ON A型神经节细胞提供甘氨酸能输入。
Amacrine cells comprise ~30 morphological types in the mammalian retina. The synaptic connectivity and function of a few GABAergic wide-field amacrine cells have recently been studied, however, with the exception of the rod pathway-specific AII amacrine cell the connectivity of glycinergic small-field amacrine cells has not been investigated in the mouse retina. Here, we studied the morphology and connectivity pattern of the small-field A8 amacrine cell. A8 cells in mouse retina are bistratified with lobular processes in the ON sublamina and arboreal dendrites in the OFF sublamina of the inner plexiform layer. The distinct bistratified morphology was first visible at postnatal day 8, reaching the adult shape at P13, around eye opening. The connectivity of A8 cells to bipolar cells and ganglion cells was studied by double and triple immunolabeling experiments using various cell markers combined with synaptic markers. Our data suggest that A8 amacrine cells receive glutamatergic input from both OFF and ON cone bipolar cells. Furthermore, A8 cells are coupled to ON cone bipolar cells by gap junctions, and provide inhibitory input via glycine receptor (GlyR) subunit α1 to OFF cone bipolar cells and to ON A-type ganglion cells. Measurements of spontaneous glycinergic postsynaptic currents and GlyR immunolabeling revealed that A8 cells express GlyRs containing the α2 subunit. Taken together, the bistratified A8 cell makes very similar synaptic contacts with cone bipolar cells as the rod pathway-specific AII amacrine cell. However, unlike AII cells, A8 amacrine cells provide glycinergic input to ON A-type ganglion cells.
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影响因子：
2.5
作者：
HENDRICKSON, AE;KOONTZ, MA;GOEBEL, DJ
通讯作者：
GOEBEL, DJ
影响因子：
2.5
作者：
Jusuf, PR;Haverkamp, S;Grünert, U
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Grünert, U
影响因子：
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WASSLE, H
影响因子：
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通讯作者：
Werblin, Frank S.
影响因子：
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作者：
Fox, Michael A.;Sanes, Joshua R.
通讯作者：
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