Evaluation of monoclonal antibody-based sandwich direct ELISA (MSD-ELISA) for antigen detection of foot-and-mouth disease virus using clinical samples.
Evaluation of monoclonal antibody-based sandwich direct ELISA (MSD-ELISA) for antigen detection of foot-and-mouth disease virus using clinical samples.
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DOI：
10.1371/journal.pone.0094143
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Kanno T
中科院分区：
文献类型：
--
作者：
Morioka K;Fukai K;Sakamoto K;Yoshida K;Kanno T
建立了一种基于单克隆抗体的夹心直接ELISA （MSD-ELISA）检测口蹄疫病毒抗原的方法。本研究评估了两种检测口蹄疫病毒抗原的msd -ELISA的敏感性和特异性，并与传统的间接夹心(IS)-ELISA进行了比较。与IS-ELISA相比，msd - elisa能够在实验感染猪的唾液样本中检测抗原的时间更长。我们还利用2010年日本o型口蹄疫暴发的178份牛和猪的rt - pcr阳性现场样本，发现两种msd - elisa对每种样本的敏感性均比IS-ELISA高约7倍（P<0.01）。在各养殖场口蹄疫阳性检出率方面，msd - elisa的敏感性约为IS-ELISA的6倍（P<0.01）。虽然还需要用其他病毒株进行进一步的验证研究，但msd - elisa可以作为替代is - elisa检测口蹄疫抗原的合适方法。
A monoclonal antibody-based sandwich direct ELISA (MSD-ELISA) method was previously developed for foot-and-mouth disease (FMD) viral antigen detection. Here we evaluated the sensitivity and specificity of two FMD viral antigen detection MSD-ELISAs and compared them with conventional indirect sandwich (IS)-ELISA. The MSD-ELISAs were able to detect the antigen in saliva samples of experimentally-infected pigs for a longer term compared to the IS-ELISA. We also used 178 RT-PCR-positive field samples from cattle and pigs affected by the 2010 type-O FMD outbreak in Japan, and we found that the sensitivities of both MSD-ELISAs were about 7 times higher than that of the IS-ELISA against each sample (P<0.01). In terms of the FMD-positive farm detection rate, the sensitivities of the MSD-ELISAs were about 6 times higher than that of the IS-ELISA against each farm (P<0.01). Although it is necessary to conduct further validation study using the other virus strains, MSD-ELISAs could be appropriate as a method to replace IS-ELISA for FMD antigen detection.
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影响因子：
3.1
作者：
Mohapatra, Jajati Keshari;Subramaniam, Saravanan;Rasool, Thaha Jamal
通讯作者：
Rasool, Thaha Jamal
影响因子：
3.1
作者：
Reid, SM;Forsyth, MA;Ferris, NP
通讯作者：
Ferris, NP
影响因子：
1.2
作者：
Sakamoto, K;Kanno, T;Murakami, Y
通讯作者：
Murakami, Y
影响因子：
9.4
作者：
Morioka, Kazuki;Fukai, Katsuhiko;Sakamoto, Kenichi
通讯作者：
Sakamoto, Kenichi
影响因子：
3.8
作者：
Samuel, AR;Knowles, NJ
通讯作者：
Knowles, NJ