A microfluidic device to acquire high-magnification microphotographs of yeast cells.
A microfluidic device to acquire high-magnification microphotographs of yeast cells.
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DOI：
10.1186/1747-1028-4-5
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发表时间：
2009-03-24
期刊：
影响因子：
2.3
通讯作者：
Ohya Y
中科院分区：
文献类型：
--
作者：
Ohnuki S;Nogami S;Ohya Y
研究酵母细胞形态，以揭示细胞形态发生的分子机制，并确定细胞周期进程等其他过程的关键因素。我们最近开发了一个名为 CalMorph 的半自动图像处理程序，它使我们能够以 501 个参数作为生物性状定量分析酵母细胞形态，并揭示细胞形态表型和基因型之间的统计关系。然而，目前的半自动方法由于其通量低，特别是在手动图像获取过程中，不适合对大规模酵母突变体进行形态学分析以可靠预测基因功能。在这项研究中，我们开发了一种微流控芯片，旨在获取适合 CalMorph 图像分析的酵母细胞的连续显微图像。利用微流控芯片，成功地表征了活细胞的形态以及细胞周期中发生的形态变化。微流控芯片使我们能够比传统方法更快地获取图像。我们推测使用微流控芯片可以有效地获取大规模图像以进行酵母菌株的自动分析。
Yeast cell morphology was investigated to reveal the molecular mechanisms of cell morphogenesis and to identify key factors of other processes such as cell cycle progression. We recently developed a semi-automatic image processing program called CalMorph, which allows us to quantitatively analyze yeast cell morphology with the 501 parameters as biological traits and uncover statistical relationships between cell morphological phenotypes and genotypes. However, the current semi-automatic method is not suitable for morphological analysis of large-scale yeast mutants for the reliable prediction of gene functions because of its low-throughput especially at the manual image-acquiring process. In this study, we developed a microfluidic chip designed to acquire successive microscopic images of yeast cells suitable for CalMorph image analysis. With the microfluidic chip, the morphology of living cells and morphological changes that occur during the cell cycle were successfully characterized. The microfluidic chip enabled us to acquire the images faster than the conventional method. We speculate that the use of microfluidic chip is effective in acquiring images of large-scale for automated analysis of yeast strains.
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DOI：
10.1111/j.1467-9868.2004.00439.x
发表时间：
2004-01-01
影响因子：
5.8
作者：
Storey, JD;Taylor, JE;Siegmund, D
通讯作者：
Siegmund, D
影响因子：
56.9
作者：
Unger, MA;Chou, HP;Quake, SR
通讯作者：
Quake, SR
影响因子：
4.5
作者：
Nogami S;Ohya Y;Yvert G
通讯作者：
Yvert G
影响因子：
3.2
作者：
Kikuchi, Yo;Mizuuchi, Eri;Ohya, Yoshikazu
通讯作者：
Ohya, Yoshikazu
影响因子：
2.7
作者：
Lee, Philip J.;Helman, Noah C.;Hung, Paul J.
通讯作者：
Hung, Paul J.