Dynamics of intra-follicular glucose during luteinization of macaque ovarian follicles.
Dynamics of intra-follicular glucose during luteinization of macaque ovarian follicles.
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DOI：
10.1016/j.mce.2010.10.011
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发表时间：
2011-01-30
影响因子：
4.1
通讯作者：
Chaffin, Charles L.
中科院分区：
文献类型：
--
作者：
Brogan, Rebecca S.;MacGibeny, Margaret;Mix, Scott;Thompson, Christopher;Puttabyatappa, Muraly;VandeVoort, Catherine A.;Chaffin, Charles L.
葡萄糖对于卵母细胞的成熟和胚胎的发育很重要，而高血糖会导致严重的生殖和发育后果。然而，人们对卵巢中葡萄糖的正常生理学仍然知之甚少。本研究的目的是确定接受受控卵巢刺激方案的非人类灵长类动物在排卵期间的卵泡内葡萄糖动态。在排卵 hCG 推注之前或之后 24 小时分离卵泡液和壁颗粒细胞，并使用人颗粒叶黄素细胞系 hGL5。 hCG 后 3 小时，卵泡内葡萄糖增加，并保持在该水平，直到 12 小时，此时水平下降回 hCG 前浓度。 hCG 并未强烈改变卵泡中的丙酮酸和乳酸浓度。 hCG 后，壁颗粒细胞的己糖激酶 1 和 2 以及葡萄糖-6-磷酸脱氢酶 mRNA 表达降低，而糖原磷酸化酶（肝型）在 hCG 后增加。 hGL5 细胞对葡萄糖的摄取延迟至刺激后 24 小时。总之，卵泡内葡萄糖在排卵刺激后增加，壁颗粒细胞似乎无法利用它，从而为卵丘-卵母细胞复合体保留了葡萄糖。
Glucose is important to the maturation of the oocyte and development of the embryo, while hyperglycemia results in profound reproductive and developmental consequences. However, the normal physiology of glucose in the ovary remains poorly understood. The goal of this study was to determine intra-follicular glucose dynamics during the periovulatory interval in non-human primates undergoing controlled ovarian stimulation protocols. Follicular fluid and mural granulosa cells were isolated before or up to 24 hr after an ovulatory hCG bolus, and the human granulosa-lutein cell line hGL5 was used. Intra-follicular glucose increased 3 hr after hCG, and remained at that level until 12 hr when levels decline back to pre-hCG concentrations. Pyruvate and lactate concentrations in the follicle were not strongly altered by hCG. Mural granulosa cell expression of hexokinase 1 and 2, and glucose-6-phosphate dehydrogenase mRNA decreased following hCG, while glycogen phosphorylase (liver form) increased following hCG. Glucose uptake by hGL5 cells was delayed until 24 hr following stimulation. In summary, intra-follicular glucose increases following an ovulatory stimulus and mural granulosa cells do not appear able to utilize it, sparing the glucose for the cumulus-oocyte complex.
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影响因子：
3.6
作者：
Downs, SM;Humpherson, PG;Leese, HJ
通讯作者：
Leese, HJ
影响因子：
4.8
作者：
Cherian-Shaw, Mary;Puttabyatappa, Muraly;Chaffin, Charles L.
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Chaffin, Charles L.
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作者：
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通讯作者：
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