Microencapsulation of islets within alginate/poly(ethylene glycol) gels cross-linked via Staudinger ligation.
Microencapsulation of islets within alginate/poly(ethylene glycol) gels cross-linked via Staudinger ligation.
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DOI：
10.1016/j.actbio.2010.07.016
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发表时间：
2011-02
影响因子：
9.7
通讯作者：
Stabler, C. L.
中科院分区：
文献类型：
--
作者：
Hall, K. K.;Gattas-Asfura, K. M.;Stabler, C. L.
采用功能化的海藻酸盐和聚乙二醇共聚物制备了高稳定性的共价连接海藻酸盐-聚乙二醇酯微球。细胞相容的Staudinger连接方案被用来化学选择性地将膦端基的聚乙二醇组成为叠氮官能化的海藻酸盐，从而得到Xalgpeg水凝胶。通过两种聚合物的共孵育，然后用钡离子对海藻酸盐进行离子交联，形成了Xalgpeg微球。研究了所制得的微球的稳定性和凝胶性能，以及这些聚合物对胰岛和胰岛细胞系的包封性。我们的数据显示，与传统的海藻酸钡(BA-Alg)微珠相比，XalgPEG微珠表现出更好的抗渗透肿胀能力，观察到的肿胀减少了5倍，并通过螯合溶液抵抗溶解。扩散和孔隙率研究发现，XAlGpeg微珠的性能与标准的BaAlg相当。我们的数据发现，Xalgpeg微球与胰岛素瘤细胞系以及大鼠和人胰岛细胞高度细胞兼容，这些细胞的存活率和功能评估与BA-Alg对照组相当。Xalgpeg水凝胶显著提高了稳定性，并表现出细胞相容性，使其成为各种组织工程应用的诱人选择。
Functionalized alginate and PEG polymers were used to generate covalently linked alginate-PEG (XAlgPEG) microbeads of high stability. The cell-compatible Staudinger ligation scheme was used to chemoselectively cross-link phosphine-terminated poly(ethylene glycol) (PEG) to azide-functionalized alginate, resulting in XAlgPEG hydrogels. XAlgPEG microbeads were formed by co-incubation of the two polymers, followed by ionic cross-linking of the alginate using barium ions. The enhanced stability and gel properties of the resulting XAlgPEG microbeads, as well as the compatibility of these polymers for the encapsulation of islets and beta cells lines, were investigated. Our data show that XAlgPEG microbeads exhibit superior resistance to osmotic swelling compared to traditional barium cross-linked alginate (Ba-Alg) beads, with a 5-fold reduction in observed swelling, as well as resistance to dissolution via chelation solution. Diffusion and porosity studies found XAlgPEG beads to exhibit properties comparable to standard Ba-Alg. Our data found XAlgPEG microbeads to be highly cell compatible with insulinoma cell lines, as well as rat and human pancreatic islets, where the viability and functional assessment of cells within XAlgPEG were comparable to Ba-Alg controls. The remarkable improved stability, as well as demonstrated cellular compatibility, of XAlgPEG hydrogels makes them an appealing option for a wide variety of tissue engineering applications.
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影响因子：
3.3
作者：
Cruise, GM;Hegre, OD;Hubbell, JA
通讯作者：
Hubbell, JA
影响因子：
3.8
作者：
HANNOUN, BJM;STEPHANOPOULOS, G
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STEPHANOPOULOS, G
影响因子：
6.2
作者：
Crescenzi, Vittorio;Cornelio, Lisa;Lamanna, Raffaele
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Lamanna, Raffaele
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作者：
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通讯作者：
Prakash, Satya
影响因子：
3.4
作者：
ESTAPE, D;GODIA, F;SOLA, C
通讯作者：
SOLA, C