Linking mechanistic and behavioral responses to sublethal esfenvalerate exposure in the endangered delta smelt; Hypomesus transpacificus (Fam. Osmeridae).
Linking mechanistic and behavioral responses to sublethal esfenvalerate exposure in the endangered delta smelt; Hypomesus transpacificus (Fam. Osmeridae).
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DOI：
10.1186/1471-2164-10-608
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发表时间：
2009-12-15
期刊：
影响因子：
4.4
通讯作者：
Werner I
中科院分区：
文献类型：
--
作者：
Connon RE;Geist J;Pfeiff J;Loguinov AV;D'Abronzo LS;Wintz H;Vulpe CD;Werner I
三角洲胡瓜鱼（Hypomesus transacificus）是一种远洋鱼类，根据美国联邦和加利福尼亚州濒危物种法案被列为濒危物种，并被认为是其栖息地范围内生态系统健康的指标，仅限于美国加州的萨克拉门托-圣华金河口。人为污染物是影响这一系统的多种压力源之一，其中，目前使用的杀虫剂是主要关注的问题。询问工具需要成功地监测污染物对三角洲冶炼的影响，并研究该物种的人口下降的潜在原因。我们已经创建了一个微阵列，以调查全基因组的潜在致病压力的影响，并应用此工具来评估拟除虫菊酯类杀虫剂esfenvalerate对幼虫三角洲胡瓜鱼的影响。使用定量PCR分析进一步研究所选基因作为分子生物标志物。在0.0625 μg·L-1的浓度下，高氰戊菊酯对三角洲胡瓜鱼幼鱼的游泳行为有显著影响，与神经肌肉活动相关的基因表达存在显著差异。与免疫反应相关的基因表达的改变，沿着细胞凋亡，氧化还原，渗透压，解毒，生长和发育似乎已经调用了高氰戊菊酯暴露。游泳障碍与乙酰化酶（ASPA）的表达显著相关，ASPA是一种参与脑细胞功能并与许多人类疾病相关的酶。选定的基因进行了调查，他们的使用作为分子生物标志物，并确定了强有力的联系之间的测量下调ASPA和观察到的行为反应暴露于环境相关的拟除虫菊酯浓度的鱼。这项研究的结果表明，微阵列技术是一种有用的方法，在濒危的，非模式生物的分子生物标志物的筛选和生成，确定特定的基因，可以直接与亚致死毒理学终点;如神经肌肉基因的表达水平的变化，导致可测量的游泳损伤。开发的微阵列成功地应用于幼鱼暴露于esfenvalerate，一个已知的污染物的萨克拉门托-圣华金河口，并允许识别特定的生物标志物，可以提供洞察三角洲胡瓜鱼人口下降的因素。
The delta smelt (Hypomesus transpacificus) is a pelagic fish species listed as endangered under both the USA Federal and Californian State Endangered Species Acts and considered an indicator of ecosystem health in its habitat range, which is limited to the Sacramento-San Joaquin estuary in California, USA. Anthropogenic contaminants are one of multiple stressors affecting this system, and among them, current-use insecticides are of major concern. Interrogative tools are required to successfully monitor effects of contaminants on the delta smelt, and to research potential causes of population decline in this species. We have created a microarray to investigate genome-wide effects of potentially causative stressors, and applied this tool to assess effects of the pyrethroid insecticide esfenvalerate on larval delta smelt. Selected genes were further investigated as molecular biomarkers using quantitative PCR analyses. Exposure to esfenvalerate affected swimming behavior of larval delta smelt at concentrations as low as 0.0625 μg.L-1, and significant differences in expression were measured in genes involved in neuromuscular activity. Alterations in the expression of genes associated with immune responses, along with apoptosis, redox, osmotic stress, detoxification, and growth and development appear to have been invoked by esfenvalerate exposure. Swimming impairment correlated significantly with expression of aspartoacylase (ASPA), an enzyme involved in brain cell function and associated with numerous human diseases. Selected genes were investigated for their use as molecular biomarkers, and strong links were determined between measured downregulation in ASPA and observed behavioral responses in fish exposed to environmentally relevant pyrethroid concentrations. The results of this study show that microarray technology is a useful approach in screening for, and generation of molecular biomarkers in endangered, non-model organisms, identifying specific genes that can be directly linked with sublethal toxicological endpoints; such as changes in expression levels of neuromuscular genes resulting in measurable swimming impairments. The developed microarrays were successfully applied on larval fish exposed to esfenvalerate, a known contaminant of the Sacramento-San Joaquin estuary, and has permitted the identification of specific biomarkers which could provide insight into the factors contributing to delta smelt population decline.
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影响因子：
11.4
作者：
Connon, Richard;Hooper, Helen L.;Callaghan, Amanda
通讯作者：
Callaghan, Amanda
DOI：
10.1073/pnas.0607817104
发表时间：
2007-01-09
影响因子：
11.1
作者：
Bitto, Eduard;Bingman, Craig A.;Phillips, George N., Jr.
通讯作者：
Phillips, George N., Jr.
影响因子：
4.5
作者：
Geist, Juergen;Werner, Inge;Leutenegger, Christian M.
通讯作者：
Leutenegger, Christian M.
DOI：
10.1016/0169-328x(94)90237-2
发表时间：
1994-06-01
期刊：
MOLECULAR BRAIN RESEARCH
影响因子：
--
作者：
BETTINI, E;PORTA, AR;MARGOLIS, FL
通讯作者：
MARGOLIS, FL
影响因子：
--
作者：
Denslow, Nancy D.;Garcia-Reyero, Natalia;Barber, David S.
通讯作者：
Barber, David S.