Dynamic content changes of cordycepin and adenosine and transcriptome in Cordyceps kyushuensis Kob at different fermentation stages

Dynamic content changes of cordycepin and adenosine and transcriptome in Cordyceps kyushuensis Kob at different fermentation stages
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九州虫草不同发酵阶段虫草素、腺苷含量及转录组的动态变化

DOI:
10.1007/s00449-021-02561-3
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发表时间:
2021-03
影响因子:
3.8
通讯作者:
Jianya Ling
Jianya Ling
中科院分区:
工程技术3区
文献类型:
--
作者:
Junyu Zhang;Tongtong Jian;Yu Zhang;Guoying Zhang;Jianya Ling

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将20%(w/w)黄芪添加到水稻培养基中培养九州黄芪。九州茶菌在菌丝期、着色期、子座形成初期和子实体期收集发酵产物。设定不同发酵阶段虫草素和腺苷含量的动态变化。黄芪大米培养基中虫草素和腺苷均在发酵第30天达到最高含量值,分别为17.31mg/g和0.94mg/g,分别是同期大米培养基的8.6倍和2.0倍。同时利用转录组学技术对九州角藻这四个时期的情况进行了分析。
20% (w/w) Astragali radix was added to the rice medium to cultivate C. kyushuensis Kob. The fermentation product was collected at mycelium stage, coloring stage, stromata-forming initial stage and fruiting body stage of C. kyushuensis Kob. The dynamic content changes of cordycepin and adenosine were setected at different fermentation stages. In the rice medium with Astragalus radix, both cordycepin and adenosine reached the highest content value on the 30th day of fermentation, 17.31mg/g and 0.94mg/g, respectively, which were 8.6 times and 2.0 times of that in rice medium at the same stage. At the same time, transcriptomics technology was used to analyze C. kyushuensis Kob during these four periods.
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