Epithelial cell coculture models for studying infectious diseases: benefits and limitations.
Epithelial cell coculture models for studying infectious diseases: benefits and limitations.
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DOI：
10.1155/2011/852419
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发表时间：
2011
影响因子：
--
通讯作者：
Ulett GC
中科院分区：
文献类型：
--
作者：
Duell BL;Cripps AW;Schembri MA;Ulett GC
无数基于使用单一谱系上皮细胞类型的体外细胞培养模型已经得到表征，并提供了对各种微生物病原体感染机制的深入了解。基于源自胃肠道、泌尿生殖系统和肺器官系统的疾病相关粘膜上皮细胞类型的多种培养模型已经描述了许多关键的宿主-病原体相互作用，这些相互作用是病毒、寄生虫和细菌疾病发病机制的基础。单谱系上皮细胞单一培养的另一种替代方法是上皮细胞与其他宿主细胞类型的共培养，它提供了更大的灵活性，并且可以克服仅基于单一细胞类型的上皮细胞培养模型的一些限制。已经描述了各种共培养模型，其将上皮细胞类型与多种其他细胞类型（包括嗜中性粒细胞、嗜酸性粒细胞、单核细胞和淋巴细胞）合并在培养物中。本文将总结当前上皮细胞共培养的模型，并将讨论上皮细胞共培养在研究传染病宿主-病原体动态方面的优点和局限性。
Countless in vitro cell culture models based on the use of epithelial cell types of single lineages have been characterized and have provided insight into the mechanisms of infection for various microbial pathogens. Diverse culture models based on disease-relevant mucosal epithelial cell types derived from gastrointestinal, genitourinary, and pulmonary organ systems have delineated many key host-pathogen interactions that underlie viral, parasitic, and bacterial disease pathogenesis. An alternative to single lineage epithelial cell monoculture, which offers more flexibility and can overcome some of the limitations of epithelial cell culture models based on only single cell types, is coculture of epithelial cells with other host cell types. Various coculture models have been described, which incorporate epithelial cell types in culture combination with a wide range of other cell types including neutrophils, eosinophils, monocytes, and lymphocytes. This paper will summarize current models of epithelial cell coculture and will discuss the benefits and limitations of epithelial cell coculture for studying host-pathogen dynamics in infectious diseases.
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影响因子：
14
作者：
Holt, Dolly J.;Chamberlain, Lisa M.;Grainger, David W.
通讯作者：
Grainger, David W.
DOI：
10.1073/pnas.89.15.7242
发表时间：
1992-08-01
影响因子：
11.1
作者：
HENDRICKS, LC;MCCLANAHAN, SL;FARQUHAR, MG
通讯作者：
FARQUHAR, MG
影响因子：
3.1
作者：
Fumagalli, O;Tall, BD;Oelschlaeger, TA
通讯作者：
Oelschlaeger, TA
影响因子：
15.9
作者：
BULL, DM;BOOKMAN, MA
通讯作者：
BOOKMAN, MA
DOI：
10.1055/s-2007-1016276
发表时间：
1998-01-01
期刊：
SEMINARS IN REPRODUCTIVE ENDOCRINOLOGY
影响因子：
--
作者：
Conway-Myers, BA
通讯作者：
Conway-Myers, BA