Osteogenetic property of a biodegradable three-dimensional macroporous hydrogel coating on titanium implants fabricated via EPD
Osteogenetic property of a biodegradable three-dimensional macroporous hydrogel coating on titanium implants fabricated via EPD
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EPD 钛种植体上生物可降解三维大孔水凝胶涂层的成骨特性
DOI：
10.1088/1748-6041/9/1/015008
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发表时间：
2014-01
影响因子：
4
通讯作者：
Wang Yining
中科院分区：
文献类型：
--
作者：
Zhou Yi;Zhang Zhen;Jiang Tao;Wang Yining
植入物与周围组织成功整合的潜力可能会在许多损害条件下受到威胁。生化表面修饰是扩大适应症范围的选择之一。我们以前已经成功地通过电泳法在钛表面制备了壳聚糖-明胶(CS/G)涂层，这可能是进一步负载功能试剂的候选材料。在本研究中，我们对CS/G涂层的组织结构、物化性能和体内生物学性能进行了研究。体外降解试验表明，添加溶菌酶的CS/G涂层在28天后失重明显。细胞培养结果表明，CS/G涂层能够支持MC3T3-E1细胞的贴壁、增殖和迁移。体内成骨行为的Micro-CT和组织形态计量学分析显示，与喷砂/酸蚀处理的种植体相比，8周和12周CS/G种植体周围有显著的新骨形成。此外，组织学评估显示，CS/G涂层在12周时大部分已降解。因此，我们得出结论，支架状CS/G涂层的三维多孔结构可能有助于成骨，并且这种涂层可以在骨愈合的早期被生物降解。
The potential for a successful integration of implants with surrounding tissue may be jeopardized in a number of compromised conditions. Biochemical surface modification is one of the choices to extend the spectrum of indications. We have previously successfully fabricated chitosan–gelatin (CS/G) coatings on a titanium surface via electrophoretic deposition, which may be promising candidates for further loading of functional agents. In this study, we have identified the microstructure, physicochemical properties and biological performance of CS/G coatings in vitro and in vivo. The in vitro degradation test indicated that CS/G coatings in the presence of lysozyme showed a significant weight loss after 28 days. The results of the cell culture exhibited that CS/G coatings could sustain MC3T3-E1 cell attachment, proliferation and migration. In vivo osteogenetic behavior evaluated by Micro-CT and histomorphometrical analysis revealed significant new bone formation around CS/G implants at 8 and 12 weeks, compared to sandblasted/acid-etched implants. Moreover, histological evaluation suggested the majority of CS/G coatings were degraded at 12 weeks. Therefore, we have concluded that the three-dimensional porous structure of scaffold-like CS/G coatings may facilitate osteogenesis and that such coatings can be biodegraded in the early bone healing process.
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影响因子：
7.5
作者：
Gerdes, Hans-Hermann;Carvalho, Raquel Negrao
通讯作者：
Carvalho, Raquel Negrao
影响因子：
14
作者：
de Jonge, Lise T.;Leeuwenburgh, Sander C. G.;Jansen, John A.
通讯作者：
Jansen, John A.
影响因子：
5.5
作者：
M. Schuler;D. Trentin;M. Textor;S. Tosatti
通讯作者：
M. Schuler;D. Trentin;M. Textor;S. Tosatti
影响因子：
4.9
作者：
Shin, H;Zygourakis, K;Mikos, AG
通讯作者：
Mikos, AG
影响因子：
4
作者：
Amini, Ashley A.;Nair, Lakshmi S.
通讯作者：
Nair, Lakshmi S.