Novel, host-restricted genotypes of Bordetella bronchiseptica associated with phocine respiratory tract isolates.
Novel, host-restricted genotypes of Bordetella bronchiseptica associated with phocine respiratory tract isolates.
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与福辛呼吸道分离株相关的支气管败血博德特氏菌的新型宿主限制基因型。
DOI：
10.1099/mic.0.000035
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Foster,Geoffrey
中科院分区：
文献类型：
--
作者：
Register,KarenB;Ivanov,YuryV;Harvill,EricT;Davison,Nick;Foster,Geoffrey
在过去25年的一系列流感病毒暴发中，支气管败血波尔德氏菌被确定为一种频繁的继发性入侵者和死亡原因。本研究的目的是评估这些暴发的海豹分离株之间的遗传多样性和宿主特异性的分子基础。对来自大西洋或北海苏格兰、英格兰或丹麦海岸的54株分离物进行MLST和PvuII核糖体分型，发现了单一的宿主限制性基因型。在里海暴发的分离株中发现了一种不同于大西洋和北海分离株的单一、新的基因型别。基于MLST序列、核型模式或全基因组SNPs的系统发育分析一致地将这两种海豹特有的基因类型归入同一主要分支，但表明每种类型都有不同的进化历史。从英格兰西南海岸的一只海豹的肠道中分离出的另一株病毒具有一种在兔子、豚鼠和猪身上发现的其他基因。为了研究寄主特异性的分子基础，利用DNA和预测的介导寄主相互作用的毒力基因的蛋白质序列，对从全基因组SNP分析中推断的北海分离物和里海分离物及其近亲进行了比较。尽管它们在系统发育上存在差异，但在两个海豹分离株之间的比较中发现的核苷酸和氨基酸替换比与密切相关的分离株相比要少。这些数据表明分离到的是OfB。与海豹呼吸道疾病相关的支气管炎包括独特的、宿主适应的和高度克隆的种群。
During a succession of phocine morbillivirus outbreaks spanning the past 25 years,Bordetella bronchisepticawas identified as a frequent secondary invader and cause of death. The goal of this study was to evaluate genetic diversity and the molecular basis for host specificity among seal isolates from these outbreaks. MLST andPvuII ribotyping of 54 isolates from Scottish, English or Danish coasts of the Atlantic or North Sea revealed a single, host-restricted genotype. A single, novel genotype, unique from that of the Atlantic and North Sea isolates, was found in isolates from an outbreak in the Caspian Sea. Phylogenetic analysis based either on MLST sequence, ribotype patterns or genome-wide SNPs consistently placed both seal-specific genotypes within the same major clade but indicates a distinct evolutionary history for each. An additional isolate from the intestinal tract of a seal on the south-west coast of England has a genotype otherwise found in rabbit, guinea pig and pig isolates. To investigate the molecular basis for host specificity, DNA and predicted protein sequences of virulence genes that mediate host interactions were used in comparisons between a North Sea isolate, a Caspian Sea isolate and each of their closest relatives as inferred from genome-wide SNP analysis. Despite their phylogenetic divergence, fewer nucleotide and amino acid substitutions were found in comparisons of the two seal isolates than in comparisons with closely related strains. These data indicate isolates ofB. bronchisepticaassociated with respiratory disease in seals comprise unique, host-adapted and highly clonal populations.
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来自不同宿主动物的支气管败血博德特氏菌分离株中 fim3 和 flaA TTGE 序列类型的分布。
影响因子：
3
作者：
A. Shina;C. Hart;Matthew D Stenton;S. Dawson;C. McCracken;S. Binns;R. Gaskell;C. Winstanley
通讯作者：
C. Winstanley
PFGE 和百日咳杆菌粘附素基因测序表明芬兰副百日咳博德特氏菌种群内的遗传变异有限。
影响因子：
3
作者：
J. Mäkinen;J. Mertsola;H. Soini;H. Arvilommi;M. Viljanen;N. Guiso;Qiushui He
通讯作者：
Qiushui He
影响因子：
3.3
作者：
Register, KB;Magyar, T
通讯作者：
Magyar, T
DOI：
10.1007/978-0-387-78885-2_10
发表时间：
2008-01-01
期刊：
BACTERIAL SIGNAL TRANSDUCTION: NETWORKS AND DRUG TARGETS
影响因子：
--
作者：
Beier, Dagmar;Gross, Roy
通讯作者：
Gross, Roy
影响因子：
9.4
作者：
GUEIRARD, P;WEBER, C;GUISO, N
通讯作者：
GUISO, N