Synthesis and characterization of photocrosslinkable gelatin and silk fibroin interpenetrating polymer network hydrogels.
Synthesis and characterization of photocrosslinkable gelatin and silk fibroin interpenetrating polymer network hydrogels.
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可光叠链链接明胶和丝绸纤维素互穿聚合物网络水凝胶的合成和表征。
DOI：
10.1016/j.actbio.2011.01.016
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发表时间：
2011-06
影响因子：
9.7
通讯作者：
Khademhosseini, Ali
中科院分区：
文献类型：
--
作者：
Xiao, Wenqian;He, Jiankang;Nichol, Jason W.;Wang, Lianyong;Hutson, Che B.;Wang, Ben;Du, Yanan;Fan, Hongsong;Khademhosseini, Ali
为了有效地修复或替换受损组织，有必要设计具有可调结构和生物力学性能的支架，以接近模拟宿主组织。本文介绍了一种以甲基丙烯酸明胶（GelMA）和丝素（SF）为基料，经顺序聚合形成的光交联互穿聚合物网络（IPN）水凝胶，具有可调的结构和生物学性能。实验结果表明，与只有GelMA交联的GelMA和半ipn水凝胶相比，GelMA和SF在互穿网络中独立交联的ipn水凝胶具有更低的溶胀率、更高的压缩模量和更低的降解率。这些差异可能是由于IPN水凝胶中结晶SF的存在导致了更高程度的整体交联。通过F-actin染色和线粒体活性分析（MTT）证实，NIH-3T3成纤维细胞很容易附着在IPN水凝胶表面，并在其上扩散和增殖。此外，利用光刻技术结合冻干技术，利用GelMA-SF IPN水凝胶制备了3D微图案和多孔微支架，进一步提高了其在各种微尺度组织工程应用中的多功能性。总的来说，这项研究介绍了一类光交联，机械坚固和可调的IPN水凝胶，可用于各种组织工程和再生医学应用。
To effectively repair or replace damaged tissues, it is necessary to design scaffolds with tunable structural and biomechanical properties that closely mimic the host tissue. In this paper, we describe a newly synthesized photocrosslinkable interpenetrating polymer network (IPN) hydrogel based on gelatin methacrylate (GelMA) and silk fibroin (SF) formed by sequential polymerization, which possesses tunable structural and biological properties. Experimental results revealed that IPNs, where both the GelMA and SF were independently crosslinked in interpenetrating networks, demonstrated a lower swelling ratio, higher compressive modulus and lower degradation rate as compared to the GelMA and semi-IPN hydrogels, where only GelMA was crosslinked. These differences were likely caused by a higher degree of overall crosslinking due to the presence of crystallized SF in the IPN hydrogels. NIH-3T3 fibroblasts readily attached to, spread, and proliferated on the surface of IPN hydrogels as demonstrated by F-actin staining and analysis of mitochondrial activity (MTT). In addition, photolithography combined with lyophilization techniques was used to fabricate 3D micropatterned and porous micro-scaffolds from GelMA-SF IPN hydrogels, furthering their versatility for use in various microscale tissue engineering applications. Overall, this study introduces a class of photocrosslinkable, mechanically robust and tunable IPN hydrogels that could be useful for various tissue engineering and regenerative medicine applications.
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影响因子：
14
作者：
Aubin H;Nichol JW;Hutson CB;Bae H;Sieminski AL;Cropek DM;Akhyari P;Khademhosseini A
通讯作者：
Khademhosseini A
影响因子：
--
作者：
Hofmann, Sandra;Knecht, Sven;Meinel, Lorenz
通讯作者：
Meinel, Lorenz
DOI：
10.1073/pnas.0801866105
发表时间：
2008-07-15
影响因子：
11.1
作者：
Du, Yanan;Lo, Edward;Khademhosseini, Ali
通讯作者：
Khademhosseini, Ali
影响因子：
14
作者：
Chen, YS;Chang, JY;Liu, BS
通讯作者：
Liu, BS
影响因子：
14
作者：
Lovett, Michael;Cannizzaro, Christopher;Kaplan, David L.
通讯作者：
Kaplan, David L.