Antidepressant-like effects of a water-soluble extract from the culture medium of Ganoderma lucidum mycelia in rats.
Antidepressant-like effects of a water-soluble extract from the culture medium of Ganoderma lucidum mycelia in rats.
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DOI：
10.1186/1472-6882-13-370
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发表时间：
2013-12-26
影响因子：
--
通讯作者：
Okazaki M
中科院分区：
文献类型：
--
作者：
Matsuzaki H;Shimizu Y;Iwata N;Kamiuchi S;Suzuki F;Iizuka H;Hibino Y;Okazaki M
灵芝是一种流行的药用蘑菇，在亚洲国家用于促进健康和长寿。此前，我们报道了灵芝菌丝体(MAK)培养液中的水溶性提取物(MAK)对脑缺血再灌注损伤具有抗氧化和脑保护作用。在此，我们评价了MAK在大鼠体内的抗抑郁和抗焦虑活性。MAK(0.3或1g/kg，P.O.)在强迫游泳、开阔场地、高架+迷宫、情境恐惧条件反射和头部抽动试验前60min给实验动物灌胃。此外，通过5-羟色胺前体5-羟基-L-色氨酸(5-HTP)或5-HT2A激动剂(±)-1-(2，5-dimethoxy-4-iodophenyl)-2-aminopropane盐酸盐(DOI)诱导的头部抽动反应，探讨了MAK抗抑郁作用的机制。在强迫游泳实验中，MAK(1g/kg)具有抗抑郁作用；在情景恐惧条件反射实验中，MAK可减轻小鼠的冰冻行为；在DOI诱导的小鼠头部抽动次数中，MAK(1g/kg)可减少DOI引起的头部抽动次数，但5-HTP不能。分别在空旷场和高架正迷宫测试中评估动物的运动或焦虑样行为时，没有观察到明显的反应。这些结果表明，MAK具有抗抑郁药样作用，这很可能是由于5-HT2A受体的拮抗作用，并对记忆依赖和/或应激诱导的焦虑具有抗焦虑作用。
Ganoderma lucidum is a popular medicinal mushroom used for promoting health and longevity in Asian countries. Previously, we reported that a water-soluble extract from a culture medium of Ganoderma lucidum mycelia (MAK) exerts antioxidative and cerebroprotective effects against ischemia–reperfusion injury in vivo. Here, we evaluated the antidepressant and anxiolytic activities of MAK in rats. MAK (0.3 or 1 g/kg, p.o.) was administered in the experimental animals 60 min before the forced swimming, open-field, elevated plus-maze, contextual fear-conditioning, and head twitch tests. Additionally, the mechanisms involved in the antidepressant-like action of MAK were investigated by the serotonin precursor 5-hydroxy-L-tryptophan (5-HTP)- or 5-HT2A agonist (±)-1-(2,5-dimethoxy-4-iodophenyl)-2-aminopropane hydrochloride (DOI)-induced head twitch responses. Treatment with MAK (1 g/kg) exhibited antidepressant-like effects in the forced swimming test, attenuated freezing behavior in the contextual fear-conditioning test, and decreased the number of head twitches induced by DOI, but not with 5-HTP. No significant response was observed in locomotion or anxiety-like behavior, when the animals were evaluated in the open-field or elevated plus-maze test, respectively. These data suggest that MAK has antidepressant-like potential, which is most likely due to the antagonism of 5-HT2A receptors, and possesses anxiolytic-like effects toward memory-dependent and/or stress-induced anxiety in rats.
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