The increasing prevalence of CPV-2c in domestic dogs in China.
The increasing prevalence of CPV-2c in domestic dogs in China.
复制标题
中国家犬中 CPV-2c 患病率不断上升
作者：
Hao X;He Y;Wang C;Xiao W;Liu R;Xiao X;Zhou P;Li S
犬细小病毒2型（Canine parvovirus type 2，CPV-2）是一种严重的犬细小病毒病，可导致犬和多种野生食肉动物的高发病率和死亡率。虽然它是一种DNA病毒，但它的进化速度特别快，基因组替换率约为10−4个替换/位点/年，接近某些RNA病毒。追踪犬中CPV-2的流行具有重要意义。方法本研究于2016 - 2019年在中国广东省进行病原学调查，涉及广州、深圳和东莞。此外，为了系统分析CPV-2在中国的流行情况，于2019年12月从NCBI核苷酸数据库下载了所有中国分离株的VP 2基因序列，并检查了CPV-2变异体的变化。结果61份样品中，经PCR检测和病毒分离，CPV-2阳性率为55.7%（34/61）。除了在犬中循环的不同变体外，还鉴定了多种变体的共感染，在某些情况下与其他犬肠道病原体共感染。本研究对国内报道的CPV-2c变异株的A5 G和Q370 R两个氨基酸位点进行了分析，并检测到多株CPV-2c变异株的P13 S和K582 N突变。最后，我们推测不同的CPV-2变异在中国的流行。从NCBI核苷酸数据库获得的VP 2基因序列来看，我国不同变异株的比例发生了变化，CPV-2c似乎增长迅速。总之，本次病原学调查表明，CPV-2在中国仍然很常见，CPV-2c的患病率正在增加。
Background Canine parvovirus type 2 (CPV-2), a serious pathogen, leads to high morbidity and mortality in dogs and several wild carnivore species. Although it is a DNA virus, it evolves particularly rapidly, with a genomic substitution rate of approximately 10−4 substitutions/site/year, close to that of some RNA viruses. Tracing the prevalence of CPV-2 in dogs is significant. Methods In this study, an aetiological survey was carried out from 2016 to 2019 in Guangdong Province, China, involving Guangzhou, Shenzhen and Dongguan. Furthermore, to systematically analyse the prevalence of CPV-2 in China, the VP2 gene sequences of all Chinese isolates were downloaded from the NCBI nucleotide database in December 2019, and changes in CPV-2 variants were examined. Results A total of 55.7% (34/61) of samples were CPV-2 positive by PCR detection and virus isolation. In addition to different variants circulating in dogs, coinfection with multiple variants was identified, as was coinfection with other canine enteric pathogens in some cases. Two previously reported amino acid sites, A5G and Q370R of CPV-2c mutants, reported in variants in China were assessed, and several CPV-2 isolates with P13S and K582N mutations were detected in this study. Finally, we speculate on the prevalence of different CPV-2 variants in China. According to the VP2 gene sequence obtained from the NCBI nucleotide database, the proportion of different variants in China has changed, and CPV-2c appears to be growing rapidly. In conclusion, this aetiology survey suggests that CPV-2 continues to be common in China and that the prevalence of CPV-2c is increasing.
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