Imaging retinal nerve fiber bundles using optical coherence tomography with adaptive optics.
Imaging retinal nerve fiber bundles using optical coherence tomography with adaptive optics.
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DOI：
10.1016/j.visres.2011.06.013
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发表时间：
2011-08-15
期刊：
影响因子：
1.8
通讯作者：
Miller, Donald T.
中科院分区：
文献类型：
--
作者：
Kocaoglu, Omer P.;Cense, Barry;Jonnal, Ravi S.;Wang, Qiang;Lee, Sangyeol;Gao, Weihua;Miller, Donald T.
早期检测视网膜神经纤维层（RNFL）轴突组织损失对于青光眼等疾病的有效治疗和管理至关重要。本研究旨在评估采用自适应光学器件的超高分辨率光学相干断层扫描 (UHR-AO-OCT) 对眼中分辨率为 3×3×3 μm3 的 RNFL 轴突束 (RNFB) 进行成像的能力。我们使用研究级 UHR-AO-OCT 系统采集了四名正常受试者和一名患有弓形视网膜神经纤维层缺损的受试者（n=5；29-62 岁）的 3°×3° 体积。从体积中提取的横截面（B 扫描）和正面（C 扫描）切片用于评估单个 RNFB 的可见性和尺寸分布。在一名受试者中，我们在七个月的时间间隔内对相同的 RNFB 进行了两次重新成像，并比较了两次成像期间的束宽度和厚度。最后，我们比较了使用 UHR-AO-OCT 和商业 OCT (Heidelberg Spectralis) 获取的弓形 RNFL 缺损的图像。在横截面和正面视图中，在 3° 视网膜偏心率的所有受试者中，个体 RNFB 都是可区分的（宽度：30-50μm，厚度：10-15μm）。在 6° 视网膜偏心率下，两个视图中 5 名受试者中的 3 名中的 RNFB 均可区分（宽度：30-45μm，厚度：20-40μm）。间隔 7 个月进行的宽度和厚度 RNFB 测量值呈强相关性 (p<0.0005)。两次测量之间的差异的平均差和标准差分别为-0.1±4.0μm（宽度）和0.3±1.5μm（厚度）。 UHR-AO-OCT 在微观视网膜的清晰度方面优于商业 OCT。据我们所知，这是在活体人眼中首次测量 RNFB 横截面。
Early detection of axonal tissue loss in retinal nerve fiber layer (RNFL) is critical for effective treatment and management of diseases such as glaucoma. This study aims to evaluate the capability of ultrahigh-resolution optical coherence tomography with adaptive optics (UHR-AO-OCT) for imaging the RNFL axonal bundles (RNFBs) with 3×3×3 μm3 resolution in the eye. We used a research-grade UHR-AO-OCT system to acquire 3°×3° volumes in four normal subjects and one subject with an arcuate retinal nerve fiber layer defect (n=5; 29–62yrs). Cross section (B-scans) and en face (C-scan) slices extracted from the volumes were used to assess visibility and size distribution of individual RNFBs. In one subject, we reimaged the same RNFBs twice over a seven month interval and compared bundle width and thickness between the two imaging sessions. Lastly we compared images of an arcuate RNFL defect acquired with UHR-AO-OCT and commercial OCT (Heidelberg Spectralis). Individual RNFBs were distinguishable in all subjects at 3° retinal eccentricity in both cross-sectional and en face views (width: 30–50μm, thickness: 10–15μm). At 6° retinal eccentricity, RNFBs were distinguishable in three of the five subjects in both views (width: 30–45μm, thickness: 20–40μm). Width and thickness RNFB measurements taken seven months apart were strongly correlated (p<0.0005). Mean difference and standard deviation of the differences between the two measurement sessions were −0.1±4.0 μm (width) and 0.3±1.5 μm (thickness). UHR-AO-OCT outperformed commercial OCT in terms of clarity of the microscopic retina. To our knowledge, these are the first measurements of RNFB cross section reported in the living human eye.
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影响因子：
3.4
作者：
Kocaoglu OP;Lee S;Jonnal RS;Wang Q;Herde AE;Derby JC;Gao W;Miller DT
通讯作者：
Miller DT
影响因子：
4.2
作者：
Greenfield, DS;Knighton, RW;Huang, XR
通讯作者：
Huang, XR
DOI：
10.2307/2987937
发表时间：
1983-01-01
期刊：
JOURNAL OF THE ROYAL STATISTICAL SOCIETY SERIES D-THE STATISTICIAN
影响因子：
--
作者：
ALTMAN, DG;BLAND, JM
通讯作者：
BLAND, JM
影响因子：
3.8
作者：
Cense, Barry;Koperda, Eric;Miller, Donald T.
通讯作者：
Miller, Donald T.
影响因子：
4.2
作者：
Costa-Cunha, Luciana V. F.;Cunha, Leonardo P.;Monteiro, Mario L. R.
通讯作者：
Monteiro, Mario L. R.