The Arabidopsis NLP7 gene regulates nitrate signaling via NRT1.1-dependent pathway in the presence of ammonium.
The Arabidopsis NLP7 gene regulates nitrate signaling via NRT1.1-dependent pathway in the presence of ammonium.
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拟南芥NLP7基因在存在铵的情况下通过NRT1.1依赖性途径调节硝酸盐信号。
DOI：
10.1038/s41598-018-20038-4
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发表时间：
2018-01-24
影响因子：
4.6
通讯作者：
Wang Y
中科院分区：
文献类型：
--
作者：
Zhao L;Zhang W;Yang Y;Li Z;Li N;Qi S;Crawford NM;Wang Y
硝酸盐不仅是一种重要的营养物质，而且是植物的一种信号分子。一些参与硝酸盐初级反应的关键分子成分主要通过正向和反向遗传学以及系统生物学被确定，然而，硝酸盐调控的许多潜在机制仍不清楚。在这项研究中，我们发现编码硝酸盐传感器和转运体（也称为CHL1和NPF6.3）的NRT1.1的表达受到nin样蛋白7 （NLP7）的调节。遗传和分子分析表明，当NH4+存在时，NLP7在硝酸盐调控中位于NRT1.1的上游，而当NH4+不存在时，NLP7在硝酸盐信号传导中独立于NRT1.1。NRT1.1在nlp7中的异位表达导致硝酸盐信号（由硝酸盐调控启动子NRP表达）、硝酸盐含量和硝酸盐还原酶活性在转基因株系中部分或完全恢复。转录组分析显示，NLP7和NRT1.1均可调控4个氮相关基因簇，包括氨基酸合成相关基因和NRT1/PTR家族成员。此外，ChIP和EMSA分析结果表明，NLP7可能结合NRT1.1启动子的特定区域。因此，在NH4+条件下，NLP7通过调控NRT1.1在硝酸盐信号传导中发挥重要作用。
Nitrate is not only an important nutrient but also a signaling molecule for plants. A few of key molecular components involved in primary nitrate responses have been identified mainly by forward and reverse genetics as well as systems biology, however, many underlining mechanisms of nitrate regulation remain unclear. In this study, we show that the expression of NRT1.1, which encodes a nitrate sensor and transporter (also known as CHL1 and NPF6.3), is modulated by NIN-like protein 7 (NLP7). Genetic and molecular analyses indicate that NLP7 works upstream of NRT1.1 in nitrate regulation when NH4+ is present, while in absence of NH4+, it functions in nitrate signaling independently of NRT1.1. Ectopic expression of NRT1.1 in nlp7 resulted in partial or complete restoration of nitrate signaling (expression from nitrate-regulated promoter NRP), nitrate content and nitrate reductase activity in the transgenic lines. Transcriptome analysis revealed that four nitrogen-related clusters including amino acid synthesis-related genes and members of NRT1/PTR family were modulated by both NLP7 and NRT1.1. In addition, ChIP and EMSA assays results indicated that NLP7 may bind to specific regions of the NRT1.1 promoter. Thus, NLP7 acts as an important factor in nitrate signaling via regulating NRT1.1 under NH4+ conditions.
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影响因子：
16.6
作者：
Medici, Anna;Marshall-Colon, Amy;Ronzier, Elsa;Szponarski, Wojciech;Wang, Rongchen;Gojon, Alain;Crawford, Nigel M.;Ruffel, Sandrine;Coruzzi, Gloria M.;Krouk, Gabriel
通讯作者：
Krouk, Gabriel
影响因子：
16.6
作者：
Marchive, Chloe;Roudier, Francois;Krapp, Anne
通讯作者：
Krapp, Anne
影响因子：
7.2
作者：
Clough, SJ;Bent, AF
通讯作者：
Bent, AF
影响因子：
4.3
作者：
Castro Marín I;Loef I;Bartetzko L;Searle I;Coupland G;Stitt M;Osuna D
通讯作者：
Osuna D
影响因子：
56.9
作者：
Lin, Rongcheng;Ding, Lei;Wang, Haiyang
通讯作者：
Wang, Haiyang