Facile synthesis, characterization, and antimicrobial activity of cellulose-chitosan-hydroxyapatite composite material: a potential material for bone tissue engineering.
Facile synthesis, characterization, and antimicrobial activity of cellulose-chitosan-hydroxyapatite composite material: a potential material for bone tissue engineering.
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DOI：
10.1002/jbm.a.34636
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发表时间：
2013-11
影响因子：
4.9
通讯作者：
Tran, Chieu D.
中科院分区：
文献类型：
--
作者：
Mututuvari, Tamutsiwa M.;Harkins, April L.;Tran, Chieu D.
羟基磷灰石（HAp）通常用作骨植入材料，因为它具有生物相容性和骨传导性。然而，HAp具有较差的流变学性质，并且其对致病微生物无活性。为了改善这些性能，我们开发了一种绿色方法来合成多功能复合材料，其包含：（1）纤维素（CEL）以赋予机械强度;（2）壳聚糖（CS）以诱导抗菌活性，从而保持无微生物的伤口部位;和（3）HAp。在该方法中，CS和CEL共溶于离子液体（IL）中，然后从水中再生。HAp随后形成原位交替浸泡[CEL+CS]复合材料在CaCl 2和Na 2 HPO 4的水溶液中。通过蒸馏[CEL+CS]的水洗液，回收至少88%的所用IL用于再利用。采用FTIR、XRD、SEM等手段对复合材料进行了表征。这些复合材料保留了其成分的理想性能。例如，通过将CEL负载从20%增加到80%，复合材料的拉伸强度提高了1.9倍。在复合材料中加入CS导致复合材料抑制革兰氏阳性菌（MRSA，S. aureus和VRE）和革兰氏阴性（E.大肠杆菌和铜绿假单胞菌）细菌。这些发现突出了[CEL+CS+HAp]复合材料作为骨组织工程支架的潜在用途。
Hydroxyapatite (HAp) is often used as a bone-implant material because it is biocompatible and osteoconductive. However, HAp possesses poor rheological properties and it is inactive against disease-causing microbes. To improve these properties, we developed a green method to synthesize multifunctional composites containing: (1) cellulose (CEL) to impart mechanical strength; (2) chitosan (CS) to induce antibacterial activity thereby maintaining a microbe-free wound site; and (3) HAp. In this method, CS and CEL were co-dissolved in an ionic liquid (IL) and then regenerated from water. HAp was subsequently formed in situ by alternately soaking [CEL+CS] composites in aqueous solutions of CaCl2 and Na2HPO4. At least 88% of IL used was recovered for reuse by distilling the aqueous washings of [CEL+CS]. The composites were characterized using FTIR, XRD and SEM. These composites retained the desirable properties of their constituents. For example, the tensile strength of the composites was enhanced 1.9X by increasing CEL loading from 20% to 80%. Incorporating CS in the composites resulted in composites which inhibited the growth of both Gram positive (MRSA, S. aureus and VRE) and Gram negative (E. coli and P. aeruginosa) bacteria. These findings highlight the potential use of [CEL+CS+HAp] composites as scaffolds in bone tissue engineering.
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影响因子：
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