Zebrafish harbor diverse intestinal macrophage populations including a subset intimately associated with enteric neural processes.
Zebrafish harbor diverse intestinal macrophage populations including a subset intimately associated with enteric neural processes.
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DOI：
10.1016/j.isci.2021.102496
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发表时间：
2021-06-25
期刊：
影响因子：
5.8
通讯作者：
Shiau CE
中科院分区：
文献类型：
--
作者：
Graves CL;Chen A;Kwon V;Shiau CE
肠道巨噬细胞对肠道健康至关重要，但在人类和小鼠系统外仍未得到充分研究。在这里，我们将斑马鱼建立为一个强大的模型，它为所有维度(前后和中心-外轴)的全肠道分析提供了优越的成像能力，以解剖胃肠道健康和疾病中的巨噬细胞生物学。我们利用高分辨率成像显示斑马鱼的肠道包含真正的肌肉和粘膜巨噬细胞，以及与肠道神经过程密切相关的令人惊讶的大亚群。有趣的是，大多数肌层巨噬细胞跨越多个肠道层，与通常局限于单层的哺乳动物巨噬细胞形成鲜明对比。利用缺乏巨噬细胞的IRF8斑马鱼，我们发现肌层但不是粘膜巨噬细胞的耗尽，而且它们可能是成人大肠运输所必需的，但在发育过程中不是。这些特征提供了对硬骨鱼肠道巨噬细胞及其与肠道神经系统从发育到成年期的联系的第一次洞察。斑马鱼的肠道肌肉和粘膜巨噬细胞与哺乳动物的肌肉和粘膜巨噬细胞相似，与肠道神经突起密切相关。斑马鱼的单个肌肉巨噬细胞跨越几个肠道层。通过IRF8消融消除选择性肌肉巨噬细胞影响肠道运输发育神经科学；免疫学
Intestinal macrophages are essential for gut health but remain understudied outside of human and mouse systems. Here, we establish zebrafish as a powerful model that provides superior imaging capabilities for whole-gut analysis along all dimensions (anterior-posterior and center-outer axes) for dissecting macrophage biology in gastrointestinal health and disease. We utilized high-resolution imaging to show that the zebrafish gut contains bona fide muscularis and mucosal macrophages, as well as surprisingly large subsets intimately associated with enteric neural processes. Interestingly, most muscularis macrophages span multiple gut layers in stark contrast to their mammalian counterparts typically restricted to a single layer. Using macrophage-deficient irf8 zebrafish, we found a depletion of muscularis but not mucosal macrophages, and that they may be dispensable for gross intestinal transit in adults but not during development. These characterizations provide first insights into intestinal macrophages and their association with the enteric nervous system from development to adulthood in teleosts. Zebrafish harbor intestinal muscularis and mucosal macrophages similar to mammals Muscularis and mucosal macrophages closely associate with enteric neural processes Individual muscularis macrophages span several gut layers in zebrafish Selective muscularis macrophage elimination by irf8 ablation affects intestinal transit Developmental neuroscience; Immunology
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DOI：
10.1084/jem.20170057
发表时间：
2018-02-05
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Bujko A;Atlasy N;Landsverk OJB;Richter L;Yaqub S;Horneland R;Øyen O;Aandahl EM;Aabakken L;Stunnenberg HG;Bækkevold ES;Jahnsen FL
通讯作者：
Jahnsen FL
影响因子：
13.5
作者：
Harry, G. Jean
通讯作者：
Harry, G. Jean
影响因子：
5.8
作者：
Dixon LJ;Barnes M;Tang H;Pritchard MT;Nagy LE
通讯作者：
Nagy LE
影响因子：
3.7
作者：
Cramer JM;Thompson T;Geskin A;LaFramboise W;Lagasse E
通讯作者：
Lagasse E
影响因子：
3.7
作者：
Earley AM;Dixon CT;Shiau CE
通讯作者：
Shiau CE