Replacement of water yam (Dioscorea alata L.) indigenous root endophytes and rhizosphere bacterial communities via inoculation with a synthetic bacterial community of dominant nitrogen-fixing bacteria.
Replacement of water yam (Dioscorea alata L.) indigenous root endophytes and rhizosphere bacterial communities via inoculation with a synthetic bacterial community of dominant nitrogen-fixing bacteria.
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DOI：
10.3389/fmicb.2023.1060239
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发表时间：
2023
影响因子：
5.2
通讯作者：
中科院分区：
文献类型：
--
作者：
含有高密度植物生长促进细菌的生物肥料作为由富营养化引起的环境问题的可持续解决方案越来越受到关注。然而，由于目前研究技术的局限性，所选择的微生物并不总是被宿主植物所偏好，从而阻止了它们被招募到原生微生物群中，或者不能诱导植物生长促进作用。为了解决这一问题，本研究对5株从淮山（学名：Aqurea阿拉塔L.）选择在山药微生物群中显示出1%或更高的优势丰度的植物，用于分析它们在用作合成细菌接种剂时的植物生长促进活性。水山药A-19植物在温室条件下种植后10周和12周接种两次。根，根际和散装土壤样品中的细菌群落的特点是使用高通量16 S rRNA扩增测序。与未接种植物相比，接种后所有细菌群落均发生了显著变化，主要是在属水平上。接种效应在定植后16周时表现明显，接种的属均占优势（前35个属）。然而，没有显着差异的任何生长参数或氮含量之间的治疗。定植后20周，接种根中寡养单胞菌的优势度下降，表明接种效果下降。有趣的是，在所有样品中，只有他生根瘤菌-新根瘤菌-副根瘤菌-根瘤菌分支占优势（>1%相对丰度），这表明与该分支相关的细菌是山药生长所必需的核心细菌。这是第一份关于在山药植物中添加合成固氮细菌群落的报告，表明天然细菌群落可以被合成细菌群落取代，接种后数周寡养单胞菌对修饰群落的影响下降。
Biofertilizers containing high-density plant growth-promoting bacteria are gaining interest as a sustainable solution to environmental problems caused by eutrophication. However, owing to the limitations of current investigative techniques, the selected microorganisms are not always preferred by the host plant, preventing recruitment into the native microbiota or failing to induce plant growth-promoting effects. To address this, five nitrogen-fixing bacteria previously isolated from water yam (Dioscorea alata L.) plants and showing dominant abundance of 1% or more in the water yam microbiota were selected for analysis of their plant growth-promoting activities when used as a synthetic bacterial inoculant. Water yam cv. A-19 plants were inoculated twice at 10 and 12 weeks after planting under greenhouse conditions. Bacterial communities in root, rhizosphere, and bulk soil samples were characterized using high-throughput 16S rRNA amplicon sequencing. Compared with non-inoculated plants, all bacterial communities were significantly altered by inoculation, mainly at the genus level. The inoculation effects were apparently found in the root communities at 16 weeks after planting, with all inoculated genera showing dominance (in the top 35 genera) compared with the control samples. However, no significant differences in any of the growth parameters or nitrogen contents were observed between treatments. At 20 weeks after planting, the dominance of Stenotrophomonas in the inoculated roots decreased, indicating a decline in the inoculation effects. Interestingly, only the Allorhizobium-Neorhizobium-Pararhizobium-Rhizobium clade was dominant (>1% relative abundance) across all samples, suggesting that bacteria related to this clade are essential core bacteria for water yam growth. This is the first report on addition of a synthetic nitrogen-fixing bacterial community in water yam plants showing that native bacterial communities can be replaced by a synthetic bacterial community, with declining in the effects of Stenotrophomonas on the modified communities several weeks after inoculation.
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影响因子：
5.2
作者：
Gómez Expósito R;Postma J;Raaijmakers JM;De Bruijn I
通讯作者：
De Bruijn I
影响因子：
48
作者：
Callahan BJ;McMurdie PJ;Rosen MJ;Han AW;Johnson AJ;Holmes SP
通讯作者：
Holmes SP
影响因子：
4.8
作者：
da Silva Araujo, Antonio Edilson;Divan Baldani, Vera Lucia;Baldani, Jose Ivo
通讯作者：
Baldani, Jose Ivo
影响因子：
2.5
作者：
Flores-Felix, Jose D.;Menendez, Esther;Rivas, Raul
通讯作者：
Rivas, Raul
影响因子：
4.8
作者：
Chao, Anne;Jost, Lou
通讯作者：
Jost, Lou