Hydroxyapitite-multiwalled carbon nanotubes nanocomposite for adhesion and electrochemical study of human osteoblast-like cells (MG-63)
Hydroxyapitite-multiwalled carbon nanotubes nanocomposite for adhesion and electrochemical study of human osteoblast-like cells (MG-63)
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用于人成骨细胞样细胞粘附和电化学研究的羟基云母-多壁碳纳米管纳米复合材料（MG-63）
DOI：
10.1016/j.electacta.2009.01.031
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发表时间：
2009-05
影响因子：
6.6
通讯作者：
中科院分区：
文献类型：
--
作者：
将羟基磷灰石-多壁碳纳米管（HA-MWCNTs）纳米复合材料制成一种新型生物相容性界面，用于人成骨样细胞（MG-63）的粘附和电化学检测。采用溶液自组装法将纳米羟基磷灰石（HA）组装到多壁碳纳米管（MWCNTs）上，制备了HA-MWCNTs纳米复合材料，并通过扫描电镜、透射电镜、X射线衍射和傅里叶变换红外光谱对其进行了表征。采用石英晶体微天平和循环伏安法研究了MG-63在纳米复合膜上的附着力。该纳米复合材料界面表现出提高的细胞固定能力和良好的生物相容性，以保持固定化活细胞的活性。将活细胞固定在HA-MWCNTs修饰玻碳电极上，观察到不可逆的阳极峰响应，该峰响应与细胞的密度和存活率呈正相关，可用于描述细胞的生长情况和评价抗肿瘤药物5-氟尿嘧啶（5-FU）对细胞的作用效果。所获得的5-FU细胞毒性结果与常规MTT法的结果一致。高度生物相容性的HA-MWCNTs纳米复合材料也有望成为一种合适的基质，用于许多其他相关哺乳动物细胞的粘附、增殖和凋亡的电化学研究。
A new biocompatible interface made up of hydroxyapitite-multiwalled carbon nanotubes (HA-MWCNTs) nanocomposite was developed for adhesion and electrochemical detection of human osteoblast-like cells (MG-63). The HA-MWCNTs nanocomposite was synthesized by self-assembling nano-hydroxyapitite onto multiwalled carbon nanotubes in solution, which was characterized by scanning electron microscopy, transmission electron microscopy, X-ray diffraction and Fourier transform infrared spectroscopy. The adhesion of the MG-63 on the nanocomposite film was studied by quartz crystal microbalance and cyclic voltammetry. The nanocomposites interface showed improved immobilization capacity for cells and good biocompatibility for preserving the activity of immobilized living cells. The living cells immobilized on an HA-MWCNTs modified glassy carbon electrode exhibited an irreversible anodic peak response being positively associated with the density and viability of cells, which was used to describe the cells growth and evaluate the effectiveness of antitumor drug 5-fluorouracil (5-FU) on the cells. The obtained 5-FU cytotoxicity results agreed well with those from conventional MTT assays. The highly biocompatible HA-MWCNTs nanocomposite is also expected to be an appropriate matrix for the electrochemical investigation of adhesion, proliferation, and apoptosis of many other relevant mammalian cells.
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