A dynamic microtubule cytoskeleton directs medial actomyosin function during tube formation.
A dynamic microtubule cytoskeleton directs medial actomyosin function during tube formation.
复制标题
DOI：
10.1016/j.devcel.2014.03.023
复制
发表时间：
2014-06-09
影响因子：
11.8
通讯作者：
Roeper, Katja
中科院分区：
文献类型：
--
作者：
Booth, Alexander J. R.;Blanchard, Guy B.;Adams, Richard J.;Roeper, Katja
细胞骨架是细胞形状变化的主要决定因素，细胞形状变化在发育过程中驱动复杂组织的形成。肌动球蛋白在组织形态发生中的重要作用已经被确定，但微管细胞骨架的作用还不清楚。在这里，我们表明，在微管发育的唾液腺在苍蝇胚胎，微管细胞骨架经历重大重排，包括90°的变化，在对齐相对于apicobasal轴，中心体附着的损失，和顶端稳定。微管细胞骨架的破坏导致注定内陷的基板细胞顶端收缩失败。我们发现，这种失败是由于失去了顶端内侧肌动球蛋白网络，其在野生型胚胎中的脉动行为驱动细胞的顶端收缩。内侧肌动球蛋白网络通过细胞连接蛋白Shot与无中心体微管束的负端相互作用，Shot的破坏也会损害顶端收缩。微管的大规模重排伴随着早期管形成微管的丢失导致管形成过程中顶端收缩的丢失在管形成过程中，顶端收缩是由脉动的内侧肌动球蛋白微管和细胞连接蛋白Shot稳定内侧肌动球蛋白细胞骨架在形态发生过程中驱动细胞形状的变化。Booth等人阐明了动态顶端内侧肌动球蛋白细胞骨架如何依赖于纵向微管束的存在。这些纤维束通过细胞连接蛋白Shot与肌动球蛋白相互作用，这种相互作用对果蝇的管形态发生很重要。
The cytoskeleton is a major determinant of cell-shape changes that drive the formation of complex tissues during development. Important roles for actomyosin during tissue morphogenesis have been identified, but the role of the microtubule cytoskeleton is less clear. Here, we show that during tubulogenesis of the salivary glands in the fly embryo, the microtubule cytoskeleton undergoes major rearrangements, including a 90° change in alignment relative to the apicobasal axis, loss of centrosomal attachment, and apical stabilization. Disruption of the microtubule cytoskeleton leads to failure of apical constriction in placodal cells fated to invaginate. We show that this failure is due to loss of an apical medial actomyosin network whose pulsatile behavior in wild-type embryos drives the apical constriction of the cells. The medial actomyosin network interacts with the minus ends of acentrosomal microtubule bundles through the cytolinker protein Shot, and disruption of Shot also impairs apical constriction. Large-scale rearrangement of microtubules accompanies early tube formation Loss of microtubules leads to loss of apical constriction during tube formation During tubulogenesis, apical constriction is driven by pulsatile medial actomyosin Microtubules and the cytolinker Shot stabilize the medial actomyosin The cytoskeleton drives cell-shape changes during morphogenesis. Booth et al. shed light on how the dynamic apical medial actomyosin cytoskeleton depends on the presence of longitudinal microtubule bundles. These bundles interact with the actomyosin through the cytolinker protein Shot, and this interaction is important for tube morphogenesis in Drosophila.
登录
查看更多内容
影响因子：
64.8
作者：
Martin, Adam C.;Kaschube, Matthias;Wieschaus, Eric F.
通讯作者：
Wieschaus, Eric F.
影响因子：
11.8
作者：
Costantini, Frank;Kopan, Raphael
通讯作者：
Kopan, Raphael
影响因子：
7.8
作者：
Bosher, Julia M;Hahn, Bum-Soo;Legouis, Renaud;Sookhareea, Satis;Weimer, Robby M;Gansmuller, Anne;Chisholm, Andrew D;Rose, Ann M;Bessereau, Jean-Louis;Labouesse, Michel
通讯作者：
Labouesse, Michel
影响因子：
2.7
作者：
Lee, Jen-Yi;Harland, Richard M.
通讯作者：
Harland, Richard M.
影响因子：
4.6
作者：
Lee, Chanjae;Scherr, Heather M.;Wallingford, John B.
通讯作者：
Wallingford, John B.