The Cap Snatching of Segmented Negative Sense RNA Viruses as a Tool to Map the Transcription Start Sites of Heterologous Co-infecting Viruses.
The Cap Snatching of Segmented Negative Sense RNA Viruses as a Tool to Map the Transcription Start Sites of Heterologous Co-infecting Viruses.
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分段负义 RNA 病毒的帽子抢夺作为绘制异源共感染病毒转录起始位点的工具
DOI：
10.3389/fmicb.2017.02519
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发表时间：
2017
影响因子：
5.2
通讯作者：
Wu Z
中科院分区：
文献类型：
--
作者：
Lin W;Qiu P;Jin J;Liu S;Ul Islam S;Yang J;Zhang J;Kormelink R;Du Z;Wu Z
病毒转录起始位点的鉴定对于理解和剖析病毒基因组转录机制具有重要意义，但这通常需要昂贵且费力的实验。许多节段性负义RNA病毒（sNSV）从多种mRNA中切割加帽前导序列，并使用这些切割的前导序列作为引物，用于在称为帽抢夺的保守过程中转录。高通量测序的最新发展使得有可能确定大多数（如果不是全部）被sNSV捕获的加帽RNA。在这里，我们表明，水稻条纹细窄病毒（RSV），植物感染sNSV，共感染烟草本塞姆与两种不同的菜豆病毒，并从他们的mRNA中抢夺加帽的前导序列。通过高通量测序确定单个RSV mRNA的5′末端，几乎所有共感染菜豆花叶病毒的mRNA的5′末端都可以被识别并定位在它们的基因组上。本研究的发现为使用sNSV的帽抢夺作为绘制病毒TSS的工具提供了支持。
Identification of the transcription start sites (TSSs) of a virus is of great importance to understand and dissect the mechanism of viral genome transcription but this often requires costly and laborious experiments. Many segmented negative-sense RNA viruses (sNSVs) cleave capped leader sequences from a large variety of mRNAs and use these cleaved leaders as primers for transcription in a conserved process called cap snatching. The recent developments in high-throughput sequencing have made it possible to determine most, if not all, of the capped RNAs snatched by a sNSV. Here, we show that rice stripe tenuivirus (RSV), a plant-infecting sNSV, co-infects Nicotiana benthamiana with two different begomoviruses and snatches capped leader sequences from their mRNAs. By determining the 5′ termini of a single RSV mRNA with high-throughput sequencing, the 5′ ends of almost all the mRNAs of the co-infecting begomoviruses could be identified and mapped on their genomes. The findings in this study provide support for the using of the cap snatching of sNSVs as a tool to map viral TSSs.
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