System-level biochip for impedance sensing and programmable manipulation of bladder cancer cells.
System-level biochip for impedance sensing and programmable manipulation of bladder cancer cells.
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DOI：
10.3390/s111111021
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
Wu YT
中科院分区：
文献类型：
--
作者：
Chuang CH;Huang YW;Wu YT
研制了一种具有多层电极和微腔阵列的介电泳芯片，用于细胞的可编程操作和阻抗测量。DEP芯片由ITO上电极、流动室、SU-8表面上的中间电极、SU-8微腔阵列和微腔底部的分布电极组成。单细胞的阻抗传感可以如下进行：首先，通过顶部和中间电极提供的负DEP力将细胞捕获在微腔阵列中;然后，通过中间和底部电极完成用于区分不同阶段的膀胱癌细胞的阻抗测量。在阻抗感测之后，使用顶部和底部电极通过负DEP力实现捕获的细胞的个体释放，以便再次收集识别的细胞。细胞操作和阻抗测量都集成在一个由基于PC的LabVIEW程序控制的系统中。在实验中，两个不同阶段的膀胱癌细胞系（III级：T24和II级：TSGH 8301）被用于演示可编程操作和阻抗传感;结果显示，低级别的膀胱癌细胞（TSGH 8301）比高级别的（T24）具有更高的阻抗。总体而言，在我们的设计中，可以通过控制电信号来容易地编程细胞的多步操作，这为芯片实验室（lab-on-a-chip，简称PLD）或微全分析系统（micro-total-analysis-system，简称Micro TAS）提供了一个很好的平台技术。
This paper develops a dielectrophoretic (DEP) chip with multi-layer electrodes and a micro-cavity array for programmable manipulations of cells and impedance measurement. The DEP chip consists of an ITO top electrode, flow chamber, middle electrode on an SU-8 surface, micro-cavity arrays of SU-8 and distributed electrodes at the bottom of the micro-cavity. Impedance sensing of single cells could be performed as follows: firstly, cells were trapped in a micro-cavity array by negative DEP force provided by top and middle electrodes; then, the impedance measurement for discrimination of different stage of bladder cancer cells was accomplished by the middle and bottom electrodes. After impedance sensing, the individual releasing of trapped cells was achieved by negative DEP force using the top and bottom electrodes in order to collect the identified cells once more. Both cell manipulations and impedance measurement had been integrated within a system controlled by a PC-based LabVIEW program. In the experiments, two different stages of bladder cancer cell lines (grade III: T24 and grade II: TSGH8301) were utilized for the demonstration of programmable manipulation and impedance sensing; as the results show, the lower-grade bladder cancer cells (TSGH8301) possess higher impedance than the higher-grade ones (T24). In general, the multi-step manipulations of cells can be easily programmed by controlling the electrical signal in our design, which provides an excellent platform technology for lab-on-a-chip (LOC) or a micro-total-analysis-system (Micro TAS).
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