Thymol Nanoemulsion: A New Therapeutic Option for Extensively Drug Resistant Foodborne Pathogens.
Thymol Nanoemulsion: A New Therapeutic Option for Extensively Drug Resistant Foodborne Pathogens.
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DOI：
10.3390/antibiotics10010025
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发表时间：
2020-12-30
期刊：
影响因子：
--
通讯作者：
Abd El-Hamid MI
中科院分区：
文献类型：
--
作者：
Bendary MM;Ibrahim D;Mosbah RA;Mosallam F;Hegazy WAH;Awad NFS;Alshareef WA;Alomar SY;Zaitone SA;Abd El-Hamid MI
食源性致病菌与严重和复杂的疾病有关。因此，这些类型的感染是公共卫生官员以及食品和乳制品行业关注的问题。关于广泛传播的多重耐药（MDR）和广泛耐药（XDR）食源性病原体，如沙门氏菌（S。肠道疾病），迫切需要新的和替代的治疗方法。因此，我们在体内方法中研究了百里酚作为百里酚纳米乳剂的稳定制剂的抗微生物、抗毒性和免疫刺激活性。值得注意的是，用2.25%百里酚纳米乳剂处理导致体重增加和饲料转化率的显著改善，以及用XDR S攻击的鸡的临床发现的严重程度和死亡率百分比的降低。肠道试验证实其显著的抗菌活性。此外，在该剂量下，百里酚纳米乳通过上调保护性细胞因子和下调XDR S而具有保护作用。肠杆菌sopB毒力基因和白细胞介素（IL）-4和IL-10等细胞因子阻碍了宿主的防御。此外，它还促进了肠道双歧杆菌的生长，从而增强了免疫系统的力量。为此，我们建议使用百里酚纳米乳剂治疗耐药食源性病原体。最后，我们建议使用2.25%百里酚纳米乳剂作为免疫功能低下个体以及兽医领域的饲料添加剂。
Foodborne pathogens have been associated with severe and complicated diseases. Therefore, these types of infections are a concern for public health officials and food and dairy industries. Regarding the wide-spread multidrug resistant (MDR) and extensively drug resistant (XDR) foodborne pathogens such as Salmonella Enteritidis (S. Enteritidis), new and alternative therapeutic approaches are urgently needed. Therefore, we investigated the antimicrobial, anti-virulence, and immunostimulant activities of a stable formulation of thymol as thymol nanoemulsion in an in vivo approach. Notably, treatment with 2.25% thymol nanoemulsion led to a pronounced improvement in the body weight gain and feed conversion ratio in addition to decreases in the severity of clinical findings and mortality percentages of challenged chickens with XDR S. Enteritidis confirming its pronounced antimicrobial activities. Moreover, thymol nanoemulsion, at this dose, had protective effects through up-regulation of the protective cytokines and down-regulation of XDR S. Enteritidis sopB virulence gene and interleukins (IL)-4 and IL-10 cytokines as those hinder the host defenses. Furthermore, it enhanced the growth of gut Bifidobacteria species, which increases the strength of the immune system. For that, we suggested the therapeutic use of thymol nanoemulsion against resistant foodborne pathogens. Finally, we recommended the use of 2.25% thymol nanoemulsion as a feed additive for immunocompromised individuals as well as in the veterinary fields.
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DOI：
10.1155/2014/651593
发表时间：
2014
期刊：
Evidence-based complementary and alternative medicine : eCAM
影响因子：
--
作者：
Bilia AR;Guccione C;Isacchi B;Righeschi C;Firenzuoli F;Bergonzi MC
通讯作者：
Bergonzi MC
DOI：
10.3390/antibiotics9100667
发表时间：
2020-10-03
期刊：
Antibiotics (Basel, Switzerland)
影响因子：
--
作者：
Abd El-Hamid MI;Y El-Naenaeey ES;M Kandeel T;Hegazy WAH;Mosbah RA;Nassar MS;Bakhrebah MA;Abdulaal WH;Alhakamy NA;Bendary MM
通讯作者：
Bendary MM
影响因子：
5.2
作者：
Abd El-Hamid, Marwa, I;Abd El-Aziz, Norhan K.;Bendary, Mahmoud M.
通讯作者：
Bendary, Mahmoud M.
DOI：
10.1111/j.1365-2672.1994.tb03443.x
发表时间：
1994-10-01
期刊：
JOURNAL OF APPLIED BACTERIOLOGY
影响因子：
--
作者：
GIBSON, GR;WANG, X
通讯作者：
WANG, X
影响因子：
4
作者：
Awad, N. F. S.;Abd El-Hamid, M. I.;Mahmoud, H. I.
通讯作者：
Mahmoud, H. I.