Protein dielectrophoresis: advances, challenges, and applications.
Protein dielectrophoresis: advances, challenges, and applications.
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DOI：
10.1002/elps.201200482
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发表时间：
2013-04
期刊：
影响因子：
2.9
通讯作者：
Ros, Alexandra
中科院分区：
文献类型：
--
作者：
Nakano, Asuka;Ros, Alexandra
蛋白质电泳（DEP）在生物分析中作为一种操作、分离、预浓缩和分离方法，以及作为纳米技术应用的操作工具，具有重要的潜力。DEP蛋白的首次展示是在近20年前报道的。从那时起，各种实验的实现，以操纵蛋白质的DEP以及更有针对性的应用，利用蛋白质DEP已被证明。本文综述了蛋白质DEP捕获和聚焦的实验研究及其在分离、分子图谱、生物探针和生物传感器等方面的具体应用。虽然描述蛋白质DEP的综合理论模型仍然缺乏，但我们也试图提供影响蛋白质DEP的因素的概述，并与目前可用的理论模型相关。我们进一步指出了过去用于研究大约20种蛋白质的实验条件的变化以及蛋白质分子结构对DEP响应的影响。
Protein dielectrophoresis (DEP) has the potential to play an important role as a manipulation, fractionation, pre-concentration and separation method in bioanalysis and as manipulation tool for nanotechnological applications. The first demonstrations of protein DEP have been reported almost twenty years ago. Since then various experimental realizations to manipulate proteins by DEP as well as more targeted applications employing protein DEP have been demonstrated. This review summarizes the experimental studies in the field of protein DEP trapping and focusing as well as specific applications in separation, molecular patterning, on bioprobes and biosensors. While a comprehensive theoretical model describing protein DEP is still lacking we also attempt to provide an overview of the factors influencing protein DEP and relate to currently available theoretical models. We further point out the variations in experimental conditions used in the past to study the somewhat 20 proteins as well as the implications of protein molecular structure to the DEP response.
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影响因子：
15.6
作者：
DUKHIN, SS;SHILOV, VN
通讯作者：
SHILOV, VN
影响因子：
10.8
作者：
Asokan, SB;Jawerth, L;Superfine, R
通讯作者：
Superfine, R
影响因子：
3.2
作者：
Henning, Anja;Bier, Frank. F.;Hoelzel, Ralph
通讯作者：
Hoelzel, Ralph
DOI：
10.1016/s0165-022x(97)00033-x
发表时间：
1997-09-25
影响因子：
--
作者：
Green, NG;Morgan, H;Milner, JJ
通讯作者：
Milner, JJ
影响因子：
9.9
作者：
Ermolina, I;Morgan, H
通讯作者：
Morgan, H