Influenza A virus infection impacts systemic microbiota dynamics and causes quantitative enteric dysbiosis.
Influenza A virus infection impacts systemic microbiota dynamics and causes quantitative enteric dysbiosis.
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DOI：
10.1186/s40168-017-0386-z
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发表时间：
2018-01-10
期刊：
影响因子：
15.5
通讯作者：
Schmolke M
中科院分区：
文献类型：
--
作者：
Yildiz S;Mazel-Sanchez B;Kandasamy M;Manicassamy B;Schmolke M
微生物区系的完整性对于越来越多的生理过程至关重要。因此，微生物区系动态平衡的破坏与多种病理状态相关。重要的是，共生微生物群提供了一种屏障，可能是通过直接竞争来抵御入侵的细菌病原体。病毒感染对宿主微生物区系组成和动态的影响还知之甚少。甲型流感病毒(IAV)是引起急性感染的常见呼吸道病原体。在这里，我们展示了在亚致死性IAV感染小鼠模型中呼吸道和肠道微生物区系的动态变化。使用16S rRNA基因特异性下一代测序和qPCR以及细菌器官内容物培养相结合的方法，我们发现了身体部位特异性和瞬时微生物区系反应。在下呼吸道，我们只观察到微生物区系组成的轻微质变。在IAV感染后没有检测到对细菌定植的定量影响，尽管有强大的抗菌宿主反应和对细菌双重感染的敏感性增加。相反，在肠道中，IAV导致细菌含量的强劲枯竭，粘液层完整性的破坏，以及Paneth细胞中更高水平的抗菌肽。作为IAV介导的微生物区系枯竭的功能结果，我们证明在鼠伤寒沙门氏菌双重感染模型中，小肠更容易受到细菌病原体的入侵。我们首次以定性和定量的方式展示了IAV感染对下呼吸道和肠道微生物区系的影响。相对16S rRNA基因下一代测序(NGS)和标准化的16S rRNA基因特异性qPCR的差异突出了定性和定量相结合的方法对正确分析器官相关微生物群落组成的重要性。短暂诱导的微生物失调强调了微生物群落对急性感染影响的总体稳定性。然而，在很短的时间内，特定的生态位可能会失去微生物群的屏障，仍然容易受到细菌的入侵。本文的在线版本(10.1186/s401680170386-z)包含补充材料，可供授权用户使用。
Microbiota integrity is essential for a growing number of physiological processes. Consequently, disruption of microbiota homeostasis correlates with a variety of pathological states. Importantly, commensal microbiota provide a shield against invading bacterial pathogens, probably by direct competition. The impact of viral infections on host microbiota composition and dynamics is poorly understood. Influenza A viruses (IAV) are common respiratory pathogens causing acute infections. Here, we show dynamic changes in respiratory and intestinal microbiota over the course of a sublethal IAV infection in a mouse model. Using a combination of 16S rRNA gene-specific next generation sequencing and qPCR as well as culturing of bacterial organ content, we found body site-specific and transient microbiota responses. In the lower respiratory tract, we observed only minor qualitative changes in microbiota composition. No quantitative impact on bacterial colonization after IAV infection was detectable, despite a robust antimicrobial host response and increased sensitivity to bacterial super infection. In contrast, in the intestine, IAV induced robust depletion of bacterial content, disruption of mucus layer integrity, and higher levels of antimicrobial peptides in Paneth cells. As a functional consequence of IAV-mediated microbiota depletion, we demonstrated that the small intestine is rendered more susceptible to bacterial pathogen invasion, in a Salmonella typhimurium super infection model. We show for the first time the consequences of IAV infection for lower respiratory tract and intestinal microbiobiota in a qualitative and quantitative fashion. The discrepancy of relative 16S rRNA gene next-generation sequencing (NGS) and normalized 16S rRNA gene-specific qPCR stresses the importance of combining qualitative and quantitative approaches to correctly analyze composition of organ associated microbial communities. The transiently induced dysbiosis underlines the overall stability of microbial communities to effects of acute infection. However, during a short-time window, specific ecological niches might lose their microbiota shield and remain vulnerable to bacterial invasion. The online version of this article (10.1186/s40168-017-0386-z) contains supplementary material, which is available to authorized users.
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DOI：
10.1038/nrmicro3552
发表时间：
2016-01
期刊：
Nature reviews. Microbiology
影响因子：
--
作者：
Donaldson GP;Lee SM;Mazmanian SK
通讯作者：
Mazmanian SK
影响因子：
8.3
作者：
Dickson, Robert P.;Erb-Downward, John R.;Curtis, Jeffrey L.
通讯作者：
Curtis, Jeffrey L.
DOI：
10.1038/ismej.2012.8
发表时间：
2012-08
期刊：
The ISME journal
影响因子：
--
作者：
通讯作者：
--
DOI：
10.1128/aem.16.4.588-594.1968
发表时间：
1968-01-01
期刊：
APPLIED MICROBIOLOGY
影响因子：
--
作者：
GAUSH, CR;SMITH, TF
通讯作者：
SMITH, TF
影响因子：
4.2
作者：
Aly, AbdelRahman Mahmoud;Adel, AbdelReheem;Aziz, Ramy K.
通讯作者：
Aziz, Ramy K.