Differential pulmonary effects of wintertime California and China particulate matter in healthy young mice.
Differential pulmonary effects of wintertime California and China particulate matter in healthy young mice.
复制标题
冬季加利福尼亚和中国颗粒物在健康的年轻小鼠中的较差肺作用。
DOI：
10.1016/j.toxlet.2017.07.853
复制
发表时间：
2017-08-15
影响因子：
3.5
通讯作者：
Pinkerton KE
中科院分区：
文献类型：
--
作者：
Sun X;Wei H;Young DE;Bein KJ;Smiley-Jewell SM;Zhang Q;Fulgar CCB;Castañeda AR;Pham AK;Li W;Pinkerton KE
空气中的颗粒物（PM）是一个全球性的问题，因为暴露与不良心肺效应有关。为了更好地了解来源导向的PM毒性，在等质量的基础上，对来自世界上两个以高PM空气污染而闻名的地理位置不同的地区（中国山西省和美国加州的中央谷）的细PM（直径≤ 2.5 µm，PM2.5）的生物效应进行了比较研究。PM2.5是在山西省省会城市（太原）和加州（萨克拉门托）在冬季收集的。本研究的首要假设是测试来自两个城市地区（一个在中国，一个在加州）的相同质量的颗粒物的化学成分是否会导致健康幼鼠肺部急性毒性和炎症的显著不同影响。雄性8周龄BALB/C小鼠（n=10只/组）通过口咽抽吸单次给予50 µg溶剂、Taiyuan PM或萨克拉门托PM。24小时后处死动物以捕获暴露后的炎症峰值。进行支气管肺泡灌洗、ELISA和组织病理学以确定生物学效应，沿着PM成分的化学分析。发现萨克拉门托PM比太原PM具有更大比例的氧化有机材料。此外，与太原PM相比，萨克拉门托PM与中性粒细胞数量显著增加以及CXCL-1和TNF-α蛋白水平升高相关。研究结果表明，在同等质量的基础上，与太原PM相比，萨克拉门托PM与更大的炎症反应相关，这可能是由更高的有机碳氧化态和可能更高的铜含量驱动的。
Airborne particulate matter (PM) is a global concern because exposure is associated with adverse cardiorespiratory effects. To better understand source-orientated PM toxicity, a comparative study of the biological effects of fine PM (diameter ≤ 2.5 µm, PM2.5) from two geographically different regions of the world known for high PM air pollution, Shanxi Province in China and the Central Valley in California in the United States was conducted on an equal mass basis. PM2.5 was collected in the capital cities of Shanxi Province (Taiyuan) and California (Sacramento) during the winter season. The overarching hypothesis for this study was to test whether the chemical composition of particulate matter on an equal mass basis from two urban areas, one in China and one in California, can lead to significantly different effects of acute toxicity and inflammation in the lungs of healthy young mice. Male, 8-week old BALB/C mice (n=10/group) were given a single 50 µg dose of vehicle, Taiyuan PM or Sacramento PM by oropharyngeal aspiration. Animals were sacrificed 24 hours later to capture peak inflammation following exposure. Bronchoalveolar lavage, ELISA and histopathology were performed to determine biological effects, along with chemical analysis of PM composition. Sacramento PM was found to have a greater proportion of oxidized organic material than Taiyuan PM. Additionally, Sacramento PM was associated with significantly increased neutrophil numbers and elevated CXCL-1 and TNF-α protein levels compared to the Taiyuan PM. The findings suggest, on an equal mass basis, Sacramento PM was associated with a greater inflammatory response compared to that of Taiyuan PM that could be driven by a higher oxidized state of organic carbon and possibly greater copper content.
登录
查看更多内容
DOI：
10.1080/15287394.2013.826567
发表时间：
2013
期刊：
Journal of toxicology and environmental health. Part A
影响因子：
--
作者：
Silva RM;Teesy C;Franzi L;Weir A;Westerhoff P;Evans JE;Pinkerton KE
通讯作者：
Pinkerton KE
影响因子：
17.1
作者：
Silva, Rona M.;Doudrick, Kyle;Franzi, Lisa M.;TeeSy, Christel;Anderson, Donald S.;Wu, Zheqiong;Mitra, Somenath;Vu, Vincent;Dutrow, Gavin;Evans, James E.;Westerhoff, Paul;Van Winkle, Laura S.;Raabe, Otto G.;Pinkerton, Kent E.
通讯作者：
Pinkerton, Kent E.
影响因子：
4.4
作者：
Ge, Xinlei;Setyan, Ari;Zhang, Qi
通讯作者：
Zhang, Qi
影响因子：
2.1
作者：
Happo, M. S.;Salonen, R. O.;Hirvonen, M. -R.
通讯作者：
Hirvonen, M. -R.
影响因子：
5
作者：
Bein, K. J.;Wexler, A. S.
通讯作者：
Wexler, A. S.