Natural diversity in the model legume Medicago truncatula allows identifying distinct genetic mechanisms conferring partial resistance to Verticillium wilt.
Natural diversity in the model legume Medicago truncatula allows identifying distinct genetic mechanisms conferring partial resistance to Verticillium wilt.
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模型豆科植物截骨模型中的自然多样性允许识别赋予对叶片叶片的部分耐药性的不同遗传机制。
DOI：
10.1093/jxb/ers337
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发表时间：
2013-01
影响因子：
6.9
通讯作者：
Gentzbittel L
中科院分区：
文献类型：
--
作者：
Ben C;Toueni M;Montanari S;Tardin MC;Fervel M;Negahi A;Saint-Pierre L;Mathieu G;Gras MC;Noël D;Prospéri JM;Pilet-Nayel ML;Baranger A;Huguet T;Julier B;Rickauer M;Gentzbittel L
黄萎病是对紫花苜蓿和许多其他作物的主要威胁。以模式豆科牧草紫花苜蓿为寄主，研究了其对黄萎病的抗性和敏感性。除了提供完善的遗传资源，这种野生植物物种还能够研究对病原体的反应的生物多样性，以及疾病和共生之间可能的串扰。根接种后的症状评分和疾病曲线的建模允许评估感病和抗病品系之间的三个杂交重组系、32个品系核心群中的重组系以及受根瘤菌共生影响的突变体的感病水平。利用一株表达绿色荧光蛋白的Albo-Atrum菌株对感病植物的定殖进行了研究。侵染元宝枫的症状和定殖模式是典型的黄萎病。利用多杂交、多点设计确定了三个不同的主效数量性状基因座，表明控制黄萎病抗性的简单遗传机制似乎是由A17和DZA45.5控制的。疾病功能参数在核心种质中的变异较大。在疾病应对方面的这种生物多样性鼓励发展联系、遗传学和生态学方法。在根瘤菌共生的不同阶段，抗性品系的几个突变体对根瘤菌共生的反应受到了影响，这表明参与共生或疾病建立的机制可能有一些共同的调控控制点。
Verticillium wilt is a major threat to alfalfa (Medicago sativa) and many other crops. The model legume Medicago truncatula was used as a host for studying resistance and susceptibility to Verticillium albo-atrum. In addition to presenting well-established genetic resources, this wild plant species enables to investigate biodiversity of the response to the pathogen and putative crosstalk between disease and symbiosis. Symptom scoring after root inoculation and modelling of disease curves allowed assessing susceptibility levels in recombinant lines of three crosses between susceptible and resistant lines, in a core collection of 32 lines, and in mutants affected in symbiosis with rhizobia. A GFP-expressing V. albo-atrum strain was used to study colonization of susceptible plants. Symptoms and colonization pattern in infected M. truncatula plants were typical of Verticillium wilt. Three distinct major quantitative trait loci were identified using a multicross, multisite design, suggesting that simple genetic mechanisms appear to control Verticillium wilt resistance in M. truncatula lines A17 and DZA45.5. The disease functional parameters varied largely in lines of the core collection. This biodiversity with regard to disease response encourages the development of association genetics and ecological approaches. Several mutants of the resistant line, impaired in different steps of rhizobial symbiosis, were affected in their response to V. albo-atrum, which suggests that mechanisms involved in the establishment of symbiosis or disease might have some common regulatory control points.
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影响因子：
3.1
作者：
Bae, JinJoo;Halterman, Dennis;Jansky, Shelley
通讯作者：
Jansky, Shelley
影响因子：
5.4
作者：
Diwan, N;Fluhr, R;Tanksley, SD
通讯作者：
Tanksley, SD
影响因子：
7.4
作者：
Fradin, Emilie F.;Zhang, Zhao;Thomma, Bart P. H. J.
通讯作者：
Thomma, Bart P. H. J.
影响因子：
3.5
作者：
Djebali, Naceur;Jauneau, Alain;Jacquet, Christophe
通讯作者：
Jacquet, Christophe
影响因子：
5.4
作者：
Hamon, Celine;Baranger, Alain;Pilet-Nayel, Marie-Laure
通讯作者：
Pilet-Nayel, Marie-Laure