Epidemiology of Infection with Epstein-Barr Virus Types 1 and 2: Lessons from the Study of a T-Cell-Immunocompromised Hemophilic Cohort
Epidemiology of Infection with Epstein-Barr Virus Types 1 and 2: Lessons from the Study of a T-Cell-Immunocompromised Hemophilic Cohort
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1 型和 2 型 Epstein-Barr 病毒感染的流行病学：T 细胞免疫功能低下的血友病队列研究的教训
作者：
Q. Yao;D. Croom;R. Tierney;G. Habeshaw;J. Wilde;F. Hill;C. Conlon;A. Rickinson
与早期关于一般高加索人群中EB病毒（EBV）携带的研究形成明显对比的是，从人类免疫缺陷病毒（HIV）阳性男性同性恋人群中分离的体外病毒显示出频繁的多种EBV感染的例子，2型EBV株的总体流行率超过30%。在这里，我们想知道这些发现在另一个T细胞免疫功能低下的队列，HIV阳性血友病患者中可能在多大程度上成立。居民EB病毒株被救出淋巴母细胞系来自39个这样的个人的血液和咽喉冲洗液，使用相同的病毒分离的体外协议，为同性恋群体。每个患者平均制备19个独立的细胞系，并且在每种情况下，通过基于PCR的病毒基因组分析和免疫印迹来表征驻留病毒以揭示病毒“EBNA印迹”。根据这些标准，血友病组中有相当比例（39例中有14例）携带一种以上的EBV毒株，这表明T细胞损伤确实使病毒携带者对外源性传播病毒的新毒株的再感染敏感。然而，2型EBV感染的总发病率为10%，这接近于早期对健康携带者的研究中观察到的发病率，并且大大低于HIV阳性同性恋者的发病率。我们推断，2型EB病毒在一般高加索人群中相对罕见，但在同性恋群体中已成为地方病。
ABSTRACT In apparent contrast to earlier work on Epstein-Barr virus (EBV) carriage in the general Caucasian population, in vitro virus isolations from human immunodeficiency virus (HIV)-positive male homosexual cohorts have shown frequent examples of multiple EBV infection and an overall prevalence of type 2 EBV strains exceeding 30%. Here we ask to what extent these findings might hold true in another T-cell-immunocompromised cohort, HIV-positive hemophilic patients. Resident EBV strains were rescued within lymphoblastoid cell lines derived from the blood and throat washings of 39 such individuals, using the same in vitro protocols of virus isolation as for the homosexual cohort. A mean of 19 independent cell lines was made per patient, and in each case the resident virus was characterized by PCR-based viral genomic analysis and by immunoblotting to reveal the viral “EBNAprint.” By these criteria a significant proportion (14 of 39) of the hemophilic cohort carried more than one EBV strain, suggesting that T-cell impairment does indeed sensitize virus carriers to reinfection with new strains of exogenously transmitted virus. However, the overall incidence of type 2 EBV infection was 10%, which is close to that observed in the earlier work with healthy carriers and substantially lower than that seen in HIV-positive homosexuals. We infer that type 2 EBV is relatively rare in the general Caucasian population but has become endemic in the homosexual community.
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DOI：
10.1073/pnas.89.14.6560
发表时间：
1992-07
影响因子：
11.1
作者：
D. Walling;S. Edmiston;J. Sixbey;Mohamed Abdel-Hamid;Mohamed Abdel-Hamid;Lionel Resnick;N. Raab-Traub
通讯作者：
D. Walling;S. Edmiston;J. Sixbey;Mohamed Abdel-Hamid;Mohamed Abdel-Hamid;Lionel Resnick;N. Raab-Traub
DOI：
10.1073/pnas.81.23.7632
发表时间：
1984-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
DAMBAUGH, T;HENNESSY, K;KIEFF, E
通讯作者：
KIEFF, E
患有艾滋病和中枢神经系统淋巴瘤的婴儿感染两种基因型的 Epstein-Barr 病毒。
DOI：
10.1093/infdis/153.3.601
发表时间：
1986
期刊：
The Journal of infectious diseases
影响因子：
--
作者：
Katz,BZ;Andiman,WA;Eastman,R;Martin,K;Miller,G
通讯作者：
Miller,G
人类免疫缺陷病毒相关非霍奇金淋巴瘤中的 Epstein-Barr 病毒亚型。
影响因子：
20.3
作者：
Boyle,MJ;Sewell,WA;Sculley,TB;Apolloni,A;Turner,JJ;Swanson,CE;Penny,R;Cooper,DA
通讯作者：
Cooper,DA
非人类免疫缺陷病毒相关口腔毛状白斑中的 Epstein-Barr 病毒共感染和重组。
DOI：
10.1093/infdis/171.5.1122
发表时间：
1995
期刊：
The Journal of infectious diseases
影响因子：
--
作者：
Walling,DM;Clark,NM;Markovitz,DM;Frank,TS;Braun,DK;Eisenberg,E;Krutchkoff,DJ;Felix,DH;Raab-Traub,N
通讯作者：
Raab-Traub,N