Proteomic analysis revealed nitrogen-mediated metabolic, developmental, and hormonal regulation of maize (Zea mays L.) ear growth.
Proteomic analysis revealed nitrogen-mediated metabolic, developmental, and hormonal regulation of maize (Zea mays L.) ear growth.
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蛋白质组学分析揭示了氮介导的玉米 (Zea mays L.) 穗生长的代谢、发育和激素调节
DOI：
10.1093/jxb/ers187
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发表时间：
2012-09
影响因子：
6.9
通讯作者：
Li X
中科院分区：
文献类型：
--
作者：
Liao C;Peng Y;Ma W;Liu R;Li C;Li X
优化氮素供应对于实现玉米高产至关重要。氮素缺乏会显著降低籽粒产量，而氮素过量会降低氮素利用效率，但不会显著提高产量。然而，潜在的蛋白质组学机制仍然知之甚少。田间试验结果表明，缺氮显著降低了穗部大小，降低了穗轴和籽粒的干物质积累，直接导致籽粒产量显著下降。进一步分析了不同施氮水平下吐丝期幼穗氮含量、生物量积累和蛋白质组的变化。缺氮显著降低玉米幼穗氮含量和生物量积累。蛋白质组学分析鉴定出47个蛋白质在不同氮素处理下幼穗中有显著差异积累。18种蛋白质对其他非生物和生物胁迫也有响应，表明氮营养失衡引发了普遍的胁迫反应。重要的是，24个蛋白质参与调节激素代谢和功能，穗发育和C/N代谢在年轻的耳朵，表明氮营养对穗生长和籽粒产量在蛋白质组水平的深刻影响。
Optimal nitrogen (N) supply is critical for achieving high grain yield of maize. It is well established that N deficiency significantly reduces grain yield and N oversupply reduces N use efficiency without significant yield increase. However, the underlying proteomic mechanism remains poorly understood. The present field study showed that N deficiency significantly reduced ear size and dry matter accumulation in the cob and grain, directly resulting in a significant decrease in grain yield. The N content, biomass accumulation, and proteomic variations were further analysed in young ears at the silking stage under different N regimes. N deficiency significantly reduced N content and biomass accumulation in young ears of maize plants. Proteomic analysis identified 47 proteins with significant differential accumulation in young ears under different N treatments. Eighteen proteins also responded to other abiotic and biotic stresses, suggesting that N nutritional imbalance triggered a general stress response. Importantly, 24 proteins are involved in regulation of hormonal metabolism and functions, ear development, and C/N metabolism in young ears, indicating profound impacts of N nutrition on ear growth and grain yield at the proteomic level.
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影响因子：
56.9
作者：
BRZOBOHATY, B;MOORE, I;PALME, K
通讯作者：
PALME, K
影响因子：
7.4
作者：
CHRISTIE, PJ;HAHN, M;WALBOT, V
通讯作者：
WALBOT, V
影响因子：
7.4
作者：
Benedetti, CE;Costa, CL;Arruda, P
通讯作者：
Arruda, P
影响因子：
7.4
作者：
CARPENTER, CD;KREPS, JA;SIMON, AE
通讯作者：
SIMON, AE
影响因子：
4.1
作者：
Czjzek, M;Cicek, M;Esen, A
通讯作者：
Esen, A