Rotavirus rearranged genomic RNA segments are preferentially packaged into viruses despite not conferring selective growth advantage to viruses.
Rotavirus rearranged genomic RNA segments are preferentially packaged into viruses despite not conferring selective growth advantage to viruses.
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DOI：
10.1371/journal.pone.0020080
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Garbarg-Chenon A
中科院分区：
文献类型：
--
作者：
Troupin C;Schnuriger A;Duponchel S;Deback C;Schnepf N;Dehee A;Garbarg-Chenon A
轮状病毒（RV）基因组由11个双链RNA片段组成。有时，RNA片段的部分序列重复导致重排的RNA片段。为了说明重排的影响，将具有重排片段7、11或两者的人RV的复制效率与同源人野生型RV（wt-RV）和牛wt-RV菌株RF的复制效率进行比较。根据病毒生长曲线判断，基因组重排的轮状病毒（r-RV）与同源wt-RV相比没有选择性生长优势。相比之下，在竞争性实验期间，r-RV相对于wt-RV被选择（即r-RV和wt-RV之间的混合感染，随后在细胞培养物中连续传代）。此外，当在具有明显生长优势的人r-RV和牛wt-RV株RF之间进行竞争性实验时，重排片段7、11或两者总是在病毒后代中分离，即使当以1 r-RV与100 wt-RV的MOI比进行混合感染时也是如此。最后，产生了从人r-RV遗传了重排片段7的牛重组病毒。尽管用重排片段7取代wt并不产生任何生长优势，但在由牛重组体r-RV和wt-RV混合感染产生的病毒后代中选择重排片段，即使MOI比为1 r-RV与107 wt-RV。在细胞培养中，r-RV相对于wt-RV缺乏选择性生长优势，这表明在病毒后代中重排片段相对于其标准对应物优先包装的机制。
The rotavirus (RV) genome consists of 11 double-stranded RNA segments. Sometimes, partial sequence duplication of an RNA segment leads to a rearranged RNA segment. To specify the impact of rearrangement, the replication efficiencies of human RV with rearranged segments 7, 11 or both were compared to these of the homologous human wild-type RV (wt-RV) and of the bovine wt-RV strain RF. As judged by viral growth curves, rotaviruses with a rearranged genome (r-RV) had no selective growth advantage over the homologous wt-RV. In contrast, r-RV were selected over wt-RV during competitive experiments (i.e mixed infections between r-RV and wt-RV followed by serial passages in cell culture). Moreover, when competitive experiments were performed between a human r-RV and the bovine wt-RV strain RF, which had a clear growth advantage, rearranged segments 7, 11 or both always segregated in viral progenies even when performing mixed infections at an MOI ratio of 1 r-RV to 100 wt-RV. Lastly, bovine reassortant viruses that had inherited a rearranged segment 7 from human r-RV were generated. Although substitution of wt by rearranged segment 7 did not result in any growth advantage, the rearranged segment was selected in the viral progenies resulting from mixed infections by bovine reassortant r-RV and wt-RV, even for an MOI ratio of 1 r-RV to 107 wt-RV. Lack of selective growth advantage of r-RV over wt-RV in cell culture suggests a mechanism of preferential packaging of the rearranged segments over their standard counterparts in the viral progeny.
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