Recruitment of fat cell precursors during high fat diet in C57BL/6J mice is fat depot specific.
Recruitment of fat cell precursors during high fat diet in C57BL/6J mice is fat depot specific.
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DOI：
10.1002/oby.20671
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发表时间：
2014-04
期刊：
影响因子：
--
通讯作者：
Kirk-Ballard H
中科院分区：
文献类型：
--
作者：
Gawronska-Kozak B;Staszkiewicz J;Gimble JM;Kirk-Ballard H
该研究旨在通过招募生命早期标记的祖细胞到脂肪系来探索饮食对体重增加和脂肪库扩大的长期影响。新生雄性C57 BL/6 J（B6）小鼠腹腔注射BrdU。从4周龄到30周龄，它们被喂食低脂肪饮食（LFD）或高脂肪饮食（HFD）。分别于术后第10天、第4、8、16和30周行流式细胞术和免疫组化检测BrdU标记细胞。喂食HFD的小鼠比喂食LFD的小鼠重，在第16周和第30周之间记录到最显著的差异。BrdU-承载细胞显示腹股沟（iWAT），附睾（eWAT）和骨髓（BM）组织中标记细胞的百分比含量降低，无论饮食如何。然而，从接受HFD的动物收集的iWAT显示在第16周标记细胞显著增加，这与16周龄至30周龄之间腹股沟脂肪重量而非附睾脂肪重量的稳健增加一致。在B6小鼠的新生期期间，用BrdU标记的细胞在脂肪组织中持续很长一段时间，并且在iWAT而不是eWAT中的有利（致肥胖）环境中被募集到脂肪细胞谱系。
The study aims to explore long-term dietary effects on increases in body mass and fat depot enlargement through the recruitment of early in life labeled progenitor cells to the adipolineage. Neonate male C57BL/6J (B6) mice were injected intraperitoneally with BrdU. From 4 until 30 weeks of age they were fed either low fat diet (LFD) or high fat diet (HFD). BrdU-labeled cells were analyzed by flow cytometric and immunohistochemical assays after 10 days and 4, 8, 16 and 30 weeks. Mice fed HFD were heavier than mice fed LFD with the most dramatic disparity recorded between week 16 and 30. BrdU-bearing cells showed the decrease in the percentage content of labeled cells in inguinal (iWAT), epididymal (eWAT) and bone marrow (BM) tissues, regardless diets. However, iWAT collected from animals on HFD showed significant increase in labeled-cells at week 16th, which coincides with robust increase in inguinal but not epididymal fat weight between 16 and 30 weeks age. Cells labeled with BrdU during neonate life of B6 mice persist in fat tissues for long period of time and are recruited to the adipocyte lineage in a favorable (obesogenic) environment in iWAT but not in eWAT.
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影响因子：
--
作者：
FAUST, IM;JOHNSON, PR;HIRSCH, J
通讯作者：
HIRSCH, J
DOI：
10.1073/pnas.0601752103
发表时间：
2006-04-25
影响因子：
11.1
作者：
Gesta, S;Blüher, M;Kahn, CR
通讯作者：
Kahn, CR
影响因子：
4.8
作者：
Sakai, Tamon;Sakaue, Hiroshi;Kasuga, Masato
通讯作者：
Kasuga, Masato
DOI：
10.1152/ajpendo.00202.2006
发表时间：
2007-01-01
影响因子：
5.1
作者：
Tchkonia, Tamara;Lenburg, Marc;Kirkland, James L.
通讯作者：
Kirkland, James L.
影响因子：
29
作者：
Tran, Thien T.;Yamamoto, Yuji;Kahn, C. Ronald
通讯作者：
Kahn, C. Ronald