Eosinophil differentiation of human umbilical cord mononuclear cells and prolonged survival of mature eosinophils by murine EL-4 thymoma cell conditioned medium.
Eosinophil differentiation of human umbilical cord mononuclear cells and prolonged survival of mature eosinophils by murine EL-4 thymoma cell conditioned medium.
复制标题
鼠 EL-4 胸腺瘤细胞条件培养基对人脐带单核细胞的嗜酸性粒细胞分化和延长成熟嗜酸性粒细胞的存活。
DOI：
10.1016/1043-4666(91)90505-8
复制
发表时间：
1991
期刊：
影响因子：
3.8
通讯作者：
Gleich,GJ
中科院分区：
文献类型：
--
作者：
Ten,RM;Butterfield,JH;Kita,H;Weiler,DA;Fischkoff,S;Ishizaka,T;Sanderson,CJ;Gleich,GJ
测试了脐带单核细胞、HL-60细胞、选择用于嗜酸性粒细胞分化的HL-60克隆和嗜酸性粒细胞白血病细胞系EoL产生嗜酸性粒细胞过氧化物酶的能力。选择用于嗜酸性粒细胞分化的 HL-60 克隆产生嗜酸性粒细胞过氧化物酶，通过细胞染色的抗氰过氧化物酶活性来判断；然而，这些细胞在长期培养中失去了产生嗜酸性粒细胞过氧化物酶的能力。相比之下，用鼠 EL-4 条件培养基 (EL-4 CM) 刺激的人脐带血单核细胞的嗜酸性粒细胞前体定期诱导嗜酸性粒细胞蛋白合成，包括嗜酸性粒细胞过氧化物酶、主要碱性蛋白、嗜酸性粒细胞阳离子蛋白和嗜酸性粒细胞衍生的神经毒素，通过抗氰过氧化物酶和嗜酸性粒细胞衍生神经毒素进行评估。 免疫荧光染色。 EL-4 CM 的这种诱导作用是在基因转录水平或 mRNA 稳定水平，如 Northern 印迹分析中嗜酸性粒细胞过氧化物酶、主要碱性蛋白和嗜酸性粒细胞衍生神经毒素的总 mRNA 增加所示。与对照嗜酸性粒细胞相比，纯化的外周血嗜酸性粒细胞与 EL-4 CM 一起孵育 4 天，其存活率有所增加。此外，这种增强的存活率被白细胞介素 5 抗血清特异性阻断。我们的结果表明，EL-4 CM 对人脐带单核细胞和成熟嗜酸性粒细胞的影响是由于白细胞介素 5 的存在。
Umbilical cord mononuclear cells, HL-60 cells, HL-60 clones selected for eosinophil differentiation, and the eosinophil leukemia cell line EoL were tested for their ability to produce eosinophil peroxidase. HL-60 clones selected for eosinophil differentiation produced eosinophil peroxidase, as judged by staining of cells for cyanide-resistant peroxidase activity; however, these cells lost their ability to produce eosinophil peroxidase in long-term culture. In contrast, eosinophil precursors from human umbilical cord blood mononuclear cells stimulated with murine EL-4 conditioned medium (EL-4 CM) were regularly induced to eosinophil protein synthesis, including eosinophil peroxidase, major basic protein, eosinophil cationic protein, and eosinophil-derived neurotoxin, as assessed by cyanide-resistant peroxidase and immunofluorescence staining. This induction by EL-4 CM is either at the level of gene transcription or mRNA stabilization, as shown by the increase of total mRNA for eosinophil peroxidase, major basic protein, and eosinophil-derived neurotoxin by Northern blot analyses. Purified peripheral blood eosinophils incubated for 4 days with EL-4 CM had increased survival over control eosinophils. Moreover, this enhanced survival was specifically blocked by antiserum to interleukin 5. Our results suggest that the effects of EL-4 CM on human umbilical cord mononuclear cells and mature eosinophils are due to the presence of interleukin 5.
登录
查看更多内容
DOI：
10.1016/s0021-9258(18)68329-2
发表时间：
1988-09
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
M. E. Rothenberg;J L Pomerantz;W. F. Owen;S. Avraham;R. Soberman;K F Austen;R. Stevens
通讯作者：
M. E. Rothenberg;J L Pomerantz;W. F. Owen;S. Avraham;R. Soberman;K F Austen;R. Stevens
脾细胞介导的嗜酸性粒细胞和中性粒细胞巨噬细胞产生的差异调节。
影响因子：
20.3
作者：
S. Bartelmez;W. Dodge;D. Bass
通讯作者：
D. Bass
影响因子：
15.9
作者：
LOPEZ, AF;WILLIAMSON, DJ;VADAS, MA
通讯作者：
VADAS, MA
嗜酸性粒细胞分化因子 (Interleukin-5) 的分子和细胞生物学及其对人和小鼠 B 细胞的影响
影响因子：
8.7
作者：
C. Sanderson;H. Campbell;I. Young
通讯作者：
I. Young
DOI：
10.1073/pnas.84.19.6629
发表时间：
1987-10-01
影响因子：
11.1
作者：
CAMPBELL, HD;TUCKER, WQJ;YOUNG, IG
通讯作者：
YOUNG, IG