Nuclear contrast angiography: a simple method for morphological characterization of cerebral arteries.
Nuclear contrast angiography: a simple method for morphological characterization of cerebral arteries.
复制标题
核对比血管造影：脑动脉形态特征的简单方法。
DOI：
10.1016/j.brainres.2009.01.020
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发表时间：
2009
期刊：
影响因子：
2.9
通讯作者：
Wang,MichaelM
中科院分区：
文献类型：
--
作者：
Meng,He;Peng,Yezi;Hasan,Rushdee;Yu,Genggeng;Wang,MichaelM
Visualization of the cerebral vascular tree is important in experimental stroke and cerebral vascular malformation research. We describe a simple method, nuclear contrast angiography, that enables simultaneous visualization of the arterial tree and cerebral endothelial cells in rodent brain whole mounts. A mixture of latex and black ink was injected into the arterial system of rodents, resulting in high contrast demarcation of the arterial tree of the brain. This method clearly differentiates arteries from veins. We applied this method to demonstrate that 14 days of unilateral carotid artery occlusion induces increases in the caliber of (1) bilateral anterior communicating arteries, (2) bilateral anterior cerebral arteries, and (3) ipsilateral proximal middle cerebral artery of the circle of Willis. Unlike other methods, this procedure selectively stains endothelial nuclei of arteries. Thus, cerebral endothelial nuclei can be visualized, quantitated, and morphologically characterized at the same time the cortical arterial tree is delineated. This method should be useful in studies of stroke and cerebral arteriogenesis, which require the accurate assessment of both arterial diameters and endothelial cell density.
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影响因子：
5.6
作者：
SMITH, MM;ANDRESSON, OS
通讯作者：
ANDRESSON, OS
影响因子：
64.5
作者：
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通讯作者：
OSLEY, MA
影响因子：
64.5
作者：
NASMYTH, K
通讯作者：
NASMYTH, K
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影响因子：
64.8
作者：
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通讯作者：
K. Nasmyth
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DOI：
10.1073/pnas.76.10.4995
发表时间：
1979
影响因子：
11.1
作者：
S. Detke;A. Lichtler;I. Phillips;J. Stein;G. Stein
通讯作者：
G. Stein