Histogen Layers Contributing to Adventitious Bud Formation Are Determined by their Cell Division Activities.
Histogen Layers Contributing to Adventitious Bud Formation Are Determined by their Cell Division Activities.
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DOI：
10.3389/fpls.2017.01749
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发表时间：
2017
影响因子：
5.6
通讯作者：
Hosokawa M
中科院分区：
文献类型：
--
作者：
Nabeshima T;Yang SJ;Ohno S;Honda K;Deguchi A;Doi M;Tatsuzawa F;Hosokawa M
在园艺中，圣保罗州是通过不定芽繁殖的，而周周嵌合品种通常很难繁殖。然而，一些周周嵌合品种可以用不定芽繁殖，其机制尚不清楚。以条纹花品种“Kaname”、“Concord”和“Monique”为研究对象，通过组织培养研究了不定芽源植物花色分离的原因。结果表明，这些品种在L1层中分别发生了类黄酮3′、5′羟化酶（SiF3′5′h）、WDR1 （SiWDR1）和类黄酮3羟化酶（SiF3H）突变。从我们之前使用“Kaname”的研究中，所有来自不定根的花都是粉红色的，这是母植物花的表皮颜色。我们使用“Concrd”和“Monique”，不仅获得了与母株表皮颜色相同的单色植物，还获得了与母株表皮颜色相同的单色植物，以及与母株表皮颜色相同的条纹花颜色。组织学观察表明，培养14 d后表皮细胞分裂活跃，参与了“小名”叶段不定芽的形成。另一方面，在“Concord”和“Monique”中，表皮下细胞的分裂数量远高于表皮细胞，并且有时表皮下细胞参与芽的形成。此外，来自“Concord”和“Monique”的l1衍生植株的植株和叶片尺寸均小于来自表皮下层的植株。综上所述，圣保利亚的外周嵌合品种可分为两类。一类在L1层具有较高的细胞分裂活性，只能从L1衍生出单一的彩色植物作为不定芽。另一种类型在L1层细胞分裂活性较低，从包括L1在内的多个层衍生出与母本相同的条纹花状植物作为不定芽。在具有不定芽繁殖能力的周周嵌合品种中，表明了L2层细胞参与分化活性较低的L1层细胞形成不定芽的可能性。
Saintpaulia ionantha is propagated by adventitious buds in horticulture, and periclinal chimeral cultivars are usually difficult to propagate. However, some periclinal chimeral cultivars can be propagated with adventitious buds, and the mechanism of which has been unknown. Striped flower cultivars “Kaname,” “Concord,” and “Monique” were used to investigate what causes flower color separation in adventitious shoot-derived plants by tissue culture. These cultivars were revealed to have mutated flavonoid 3′, 5′ hydroxylase (SiF3′5′H), WDR1 (SiWDR1), or flavonoid 3 hydroxylase (SiF3H), respectively, in their L1 layer. From our previous study using “Kaname,” all flowers from adventitious shoots were colored pink, which was the epidermal color of mother plants' flowers. We used “Concrd” and “Monique” from which we obtained not only monochromatic-colored plants the same as the epidermal color of mother plants, but also plants with a monochromatic colored plants, same as the subepidermal color, and a striped flower color the same as mother plants. Histological observations revealed that epidermal cells divided actively at 14 d after culture and they were involved in the formation of adventitious shoots in the cultured leaf segments of “Kaname.” On the other hand, in “Concord” and “Monique,” the number of divided cells in the subepidermis was rather higher than that of epidermal cells, and subepidermal cells were sometimes involved in shoot formation. In addition, the plant and leaf size of L1-derived plants from “Concord” and “Monique” were non-vigorous and smaller than those derived from the subepidermal layer. In conclusion, periclinal chimeral cultivars of Saintpaulia can be divided into two types. One type has a high cell division activity in the L1 layer, from which only single flower-colored plants derived from L1 can be obtained as adventitious shoots. Another type has a low cell division activity in the L1 layer, from which striped flower-colored plants the same as mother plants derived from several layers including L1 can be obtained as adventitious shoots. In the periclinal chimeral cultivar capable of propagation with adventitious shoots, the possibility was shown that cells in the L2 layer could form shoots by involving cells of the L1 layer with a low division activity.
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影响因子：
1.9
作者：
ABUQAOUD, H;SKIRVIN, RM;CHEVREAU, E
通讯作者：
CHEVREAU, E
影响因子：
1.9
作者：
ESPINO, FJ;VAZQUEZ, AM
通讯作者：
VAZQUEZ, AM
影响因子：
4.2
作者：
MARCOTRIGIANO, M;GOUIN, FR
通讯作者：
GOUIN, FR
影响因子：
6.4
作者：
MURASHIGE, T;SKOOG, F
通讯作者：
SKOOG, F
影响因子：
4.3
作者：
ANDO, T;AKIYAMA, Y;YOKOI, M
通讯作者：
YOKOI, M