Effects of Prunella vulgaris on the mice immune function.
Effects of Prunella vulgaris on the mice immune function.
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DOI：
10.1371/journal.pone.0077355
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Ji G
中科院分区：
文献类型：
--
作者：
Huang R;Zhao M;Yang X;Huang J;Yang Y;Chen B;Tan J;Huang J;Li Z;Lv Y;Ji G
本研究旨在评估夏枯草（P. vulgaris）对小鼠免疫功能的影响。将小鼠随机分为1个对照组和3个治疗组，每组10只小鼠。对照组给予纯净水，治疗组口服浓度分别为0.15、0.30和0.90 g/kg BW的寻常假草提取物，持续30天。评价细胞免疫功能、非特异性免疫和体液免疫功能的变化。通过荧光激活细胞分选术 (FACS) 测定活性淋巴细胞和 T 淋巴细胞亚群。通过酶联免疫吸附测定（ELISA）测量细胞因子的某些血清浓度。结果显示，细胞免疫功能方面，与对照组相比，高剂量组足垫厚度显着增加（p<0.01），而各组脾淋巴细胞增殖能力无显着差异（p>0.05）。对于非特异性免疫，普通假单胞菌处理小鼠的NK细胞活性以剂量依赖性方式显着增加（p<0.01），中和高剂量普通假单胞菌处理小鼠的单核巨噬细胞功能显着高于对照组（p<0.05）。体液免疫方面，溶血半值（HC50）、溶血斑数和血清IgG水平无显着差异（p>0.05）。高剂量组小鼠外周血活性T、Th淋巴细胞百分率显着高于对照组（p<0.01）。四组间血清IL-1β、IL-4、IL-10、IFN-γ水平比较，差异均无统计学意义（p>0.05）。数据表明，0.90 g/kg BW 寻常苋提取物（相当于7.5 g/kg BW生药）对小鼠细胞免疫功能和非特异性免疫功能有一定影响。
The present study was designed to evaluate the effects of Prunella Vulgaris (P. vulgaris) on the immune function in mice. The mice were randomly divided into one control group and three treatment groups of 10 mice each. The control group received pure water and the treatment groups received P. vulgaris extract at concentrations of 0.15, 0.30 and 0.90 g/kg BW orally for 30 days, respectively. Changes in cell immune function, non-specific immunity and humoral immunity function were evaluated. Active lymphocytes and T lymphocyte subsets were determined by fluorescence-activated cell sorting (FACS). Certain Serum concentrations of cytokines were measured by enzyme-linked immunosorbent assay (ELISA). The results showed that, for cell immune function, compared with the control group, foot pad thickness in high dose group increased significantly (p<0.01), whereas no significant difference in the proliferative ability of splenic lymphocytes was observed among all groups (p>0.05). For non-specific immunity, NK cell activity increased significantly in a dose-dependent manner in P. vulgaris treated mice (p<0.01), mononuclear-macrophage function in medium and high dose P. vulgaris treated mice were significantly higher than that of the control group (p<0.05). For humoral immunity, no significant differences were observed in terms of the half value of hemolysis (HC50), number of hemolytic plaques and serum IgG level (p>0.05). The percentage of active T and Th lymphocytes of mice peripheral blood in high dose group were significantly higher than that of the control group (p<0.01). There was no significant difference in serum levels of IL-1β, IL-4, IL-10 and IFN-γ among all of the four groups (p>0.05). The data indicated that 0.90 g/kg BW P. vulgaris extract (equivalent to 7.5 g/kg BW crude drug) had some effect on cellular immune function and non-specific immune function in mice.
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影响因子：
6.1
作者：
Huang N;Hauck C;Yum MY;Rizshsky L;Widrlechner MP;McCoy JA;Murphy PA;Dixon PM;Nikolau BJ;Birt DF
通讯作者：
Birt DF
影响因子：
4.3
作者：
Han, Eun Hee;Choi, Jae Ho;Jeong, Hye Gwang
通讯作者：
Jeong, Hye Gwang
影响因子：
3.2
作者：
Zdarilova, A.;Svobodova, A.;Ulrichova, J.
通讯作者：
Ulrichova, J.
影响因子：
4.4
作者：
Ikeda, Yasutaka;Murakami, Akira;Ohigashi, Hajime
通讯作者：
Ohigashi, Hajime
影响因子：
2.7
作者：
Ryu, SY;Oak, MH;Kim, KM
通讯作者：
Kim, KM