Evidence for transmission of bluetongue virus serotype 26 through direct contact.
Evidence for transmission of bluetongue virus serotype 26 through direct contact.
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DOI：
10.1371/journal.pone.0096049
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Oura C
中科院分区：
文献类型：
--
作者：
Batten C;Darpel K;Henstock M;Fay P;Veronesi E;Gubbins S;Graves S;Frost L;Oura C
本研究旨在探讨蓝舌病毒26型(BTV-26)在山羊体内传播的机制。之前的一项研究调查了BTV-26在山羊中的致病性和感染动力学，意外地揭示了一只对照山羊可能是通过直接接触传播途径感染的。为了更详细地探讨BTV-26的传播机制，对3只感染BTV-26的山羊进行了实验性感染研究，其中3只山羊未被感染，但与感染的山羊直接接触，另外4只山羊与感染的山羊用金属栅栏隔开。这一屏障允许山羊在同一空域偶尔面对面接触，但饲养、浇水、采样和环境清洁是分开进行的。三只实验感染的山羊没有表现出BTV的临床症状，但检测到高水平的病毒RNA，并从它们的血液中分离出病毒。在21dpi时，从三只直接接触的山羊的血液中检测到病毒RNA，并从血液中分离到病毒，这些山羊也进行了血清转换。在整个实验过程中，四只间接屏障接触山羊没有受到感染。为了评估库蚊叮咬蚊虫的实验室模式物种的复制情况，给300多只索诺尔库蚊喂食添加了BTV-26的血粉，并孵化7天。从病毒RNA的数量推断BTV-26病毒在索诺氏库蚊个体中的传播，表明在第7天处理的昆虫中没有一种具有可传播的感染。这项研究表明，BTV-26很容易通过山羊之间的直接接触传播，在标准的孵化条件下，该毒株似乎不会在华支睾吸虫中复制。
The aim of this study was to assess the mechanisms of transmission of bluetongue virus serotype 26 (BTV-26) in goats. A previous study, which investigated the pathogenicity and infection kinetics of BTV-26 in goats, unexpectedly revealed that one control goat may have been infected through a direct contact transmission route. To investigate the transmission mechanisms of BTV-26 in more detail an experimental infection study was carried out in which three goats were infected with BTV-26, three goats were kept uninfected, but were housed in direct contact with the infected goats, and an additional four goats were kept in indirect contact separated from infected goats by metal gates. This barrier allowed the goats to have occasional face-to-face contact in the same airspace, but feeding, watering, sampling and environmental cleaning was carried out separately. The three experimentally infected goats did not show clinical signs of BTV, however high levels of viral RNA were detected and virus was isolated from their blood. At 21 dpi viral RNA was detected in, and virus was isolated from the blood of the three direct contact goats, which also seroconverted. The four indirect barrier contact goats remained uninfected throughout the duration of the experiment. In order to assess replication in a laboratory model species of Culicoides biting midge, more than 300 Culicoides sonorensis were fed a BTV-26 spiked blood meal and incubated for 7 days. The dissemination of BTV-26 in individual C. sonorensis was inferred from the quantity of virus RNA and indicated that none of the insects processed at day 7 possessed transmissible infections. This study shows that BTV-26 is easily transmitted through direct contact transmission between goats, and the strain does not seem to replicate in C. sonorensis midges using standard incubation conditions.
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影响因子：
2.2
作者：
Carpenter, S.;McArthur, C.;Mellor, P. S.
通讯作者：
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影响因子：
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作者：
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影响因子：
2.2
作者：
Menzies, F. D.;Mccullough, S. J.;Oura, C. A. L.
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