NR2B subunit of the NMDA receptor in the basolateral amygdala is necessary for the acquisition of conditioned defeat in Syrian hamsters.
NR2B subunit of the NMDA receptor in the basolateral amygdala is necessary for the acquisition of conditioned defeat in Syrian hamsters.
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DOI：
10.1016/j.bbr.2010.09.034
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发表时间：
2011-02-02
影响因子：
2.7
通讯作者：
Huhman, Kim L.
中科院分区：
文献类型：
--
作者：
Day, Diane E.;Cooper, Matthew A.;Markham, Chris M.;Huhman, Kim L.
基底外侧杏仁核（BLA）的可逆性失活会破坏条件性失败（CD）的获得和表达，条件性失败是一种条件性恐惧的行为学模型，这表明 BLA 可能是介导与社交失败体验相关的行为可塑性的神经回路的关键组成部分。我们还表明，这种效应是 N-甲基-D-天冬氨酸 (NMDA) 受体依赖性的，因为将 D,L-2-氨基-5-磷酸戊酸 (APV) 输注到 BLA 中也会损害 CD 的获得。然而，APV 是一种非选择性 NMDA 拮抗剂，因此它会破坏整个异聚受体复合物，因此很难区分 NR2A 或 NR2B 受体亚型对 CD 获得的相对贡献。然而，有充分的证据表明，杏仁核中 NMDA 受体的 NR2B 亚基对于调节与恐惧学习相关的长期增强和可塑性至关重要。本实验的目的是确定将 NR2B 亚基的选择性拮抗剂艾芬地尔输注至 BLA 中是否会阻断 CD 的获得（但不阻断表达）。在实验1中，在失败训练前立即注射艾芬地尔，当仓鼠后来与非攻击性入侵者（NAI）配对时，显着减少了顺从行为并恢复了领土攻击性。相反，在 CD 测试之前立即输注艾芬地尔未能抑制先前击败的仓鼠的顺从行为的表达。这些结果支持这样的假设：BLA 是社会失败引起的行为变化的可塑性的关键部位。
Reversible inactivation of the basolateral amygdala (BLA) disrupts the acquisition and expression of conditioned defeat (CD), an ethological model of conditioned fear, suggesting that the BLA may be a critical component of the neural circuit mediating behavioral plasticity associated with the experience of social defeat. We have also shown that this effect is N-methyl-D-aspartic acid (NMDA) receptor-dependent, because infusion of D,L-2-amino-5-phosphovalerate (APV) into the BLA also impairs the acquisition of CD. APV is a non-selective NMDA antagonist, however, thus it disrupts the entire heteromeric receptor complex, making it difficult to distinguish the relative contributions of either the NR2A or NR2B receptor subtypes on the acquisition of CD. There is ample evidence, however, that the NR2B subunit of the NMDA receptor in the amygdala is critical for mediating long-term potentiation and plasticity related to fear learning. The purpose of the present experiment was to determine whether infusion of ifenprodil, a selective antagonist of the NR2B subunit, into the BLA would block the acquisition (but not expression) of CD. In Experiment 1, infusion of ifenprodil immediately before defeat training significantly decreased submissive behaviors and restored territorial aggression when hamsters were later paired with a non-aggressive intruder (NAI). Conversely, infusion of ifenprodil immediately before CD testing failed to inhibit the expression of submissive behaviors in previously defeated hamsters. These results support the hypothesis that the BLA is a critical site for the plasticity underlying social defeat-induced changes in behavior.
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影响因子：
5.3
作者：
Rodrigues, SM;Schafe, GE;LeDoux, JE
通讯作者：
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Jasnow, AM;Davis, M;Huhman, KL
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作者：
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