Nonrandom Selection and Multiple Blood Feeding of Human Hosts by Anopheles Vectors: Implications for Malaria Transmission in Papua New Guinea.
Nonrandom Selection and Multiple Blood Feeding of Human Hosts by Anopheles Vectors: Implications for Malaria Transmission in Papua New Guinea.
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DOI：
10.4269/ajtmh.21-0210
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发表时间：
2021-09-27
期刊：
影响因子：
--
通讯作者：
Walker ED
中科院分区：
文献类型：
--
作者：
Keven JB;Katusele M;Vinit R;Rodríguez-Rodríguez D;Hetzel MW;Robinson LJ;Laman M;Karl S;Foran DR;Walker ED
按蚊对人类宿主的非随机选择和多次供血可能会加剧疟疾传播。在巴布亚新几内亚（PNG）的按蚊媒介中研究了两种吸血模式及其与疟疾流行病学的关系。在村庄收集了人类的血液样本和蚊子的血粉，并通过对 23 个微卫星和性别特异性标记进行基因分型来分析人类遗传图谱（“指纹”）。从不同人类获得的血粉频率，通过独特的基因图谱进行鉴定，符合泊松分布和负二项分布，以测试宿主选择的非随机模式。具有不止一种基因谱的血粉被归类为以多人为食的蚊子。通过将血粉基因图谱与村民的基因图谱进行匹配来确定被蚊子叮咬的人的年龄。通过对血液样本进行 PCR 检测来确定人类是否感染疟疾。结果显示，人类血液喂养的分布是非随机的，对男性和 15-30 岁个体的选择存在偏差。该年龄段的恶性疟原虫感染率较高，表明该年龄段的男性可能是疟疾寄生虫的超级传播者。在村庄中，以多人为食的蚊子比例为 6% 至 13%。这里观察到的宿主利用模式可能会放大传播并导致巴布亚新几内亚疟疾的持续存在，尽管人们努力用杀虫蚊帐抑制疟疾。对 15-30 岁男性的过度喂养凸显了针对这一人口群体采取有针对性的干预措施的重要性。
Nonrandom selection and multiple blood feeding of human hosts by Anopheles mosquitoes may exacerbate malaria transmission. Both patterns of blood feeding and their relationship to malaria epidemiology were investigated in Anopheles vectors in Papua New Guinea (PNG). Blood samples from humans and mosquito blood meals were collected in villages and human genetic profiles (“fingerprints”) were analyzed by genotyping 23 microsatellites and a sex-specific marker. Frequency of blood meals acquired from different humans, identified by unique genetic profiles, was fitted to Poisson and negative binomial distributions to test for nonrandom patterns of host selection. Blood meals with more than one genetic profiles were classified as mosquitoes that fed on multiple humans. The age of a person bitten by a mosquito was determined by matching the blood-meal genetic profile to the villagers’ genetic profiles. Malaria infection in humans was determined by PCR test of blood samples. The results show nonrandom distribution of blood feeding among humans, with biased selection toward males and individuals aged 15–30 years. Prevalence of Plasmodium falciparum infection was higher in this age group, suggesting males in this age range could be super-spreaders of malaria parasites. The proportion of mosquitoes that fed on multiple humans ranged from 6% to 13% among villages. The patterns of host utilization observed here can amplify transmission and contribute to the persistence of malaria in PNG despite efforts to suppress it with insecticidal bed nets. Excessive feeding on males aged 15–30 years underscores the importance of targeted interventions focusing on this demographic group.
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影响因子：
3.7
作者：
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