Quantum dot-based HIV capture and imaging in a microfluidic channel.
Quantum dot-based HIV capture and imaging in a microfluidic channel.
复制标题
基于量子点的HIV捕获和成像在微流体通道中。
DOI：
10.1016/j.bios.2009.06.023
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发表时间：
2009-09-15
影响因子：
12.6
通讯作者：
Demirci U
中科院分区：
文献类型：
--
作者：
Kim YG;Moon S;Kuritzkes DR;Demirci U
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Globally, over 33.2 million people who mostly live in developing countries with limited access to the appropriate medical care suffer from the human immunodeficiency virus (HIV) infection. We developed an on-chip HIV capture and imaging method using quantum dots (Qdots) from fingerprick volume (10 μl) of unprocessed HIV-infected patient whole blood in anti-gp120 antibody-immobilized microfluidic chip. Two-color Qdots (Qdot525 and Qdot655 streptavidin conjugates) were used to identify the captured HIV by simultaneous labeling the envelope gp120 glycoprotein and its high-mannose glycans. This dual-stain imaging technique using Qdots provides a new and effective tool for accurate identification of HIV particles from patient whole blood without any pre-processing. This on-chip HIV capture and imaging platform creates new avenues for point-of-care diagnostics and monitoring applications of infectious diseases.
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