Vascular endothelial growth factor and physiological compressive loading synergistically promote bone formation of tissue-engineered bone.
Vascular endothelial growth factor and physiological compressive loading synergistically promote bone formation of tissue-engineered bone.
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血管内皮生长因子和生理压缩负荷协同促进组织工程骨的骨形成。
DOI：
10.1089/ten.tea.2013.0124
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发表时间：
2013-08
期刊：
影响因子：
--
通讯作者：
Xu, Jianzhong
中科院分区：
文献类型：
--
作者：
Wu, Xuehui;Hou, Tianyong;Luo, Fei;Xing, Junchao;He, Qingyi;Jin, Huiyong;Xie, Zhao;Xu, Jianzhong
血管生成和骨生成对于骨损伤的愈合至关重要，尤其是与临界骨缺损 (CSBD) 相关的骨损伤。骨组织工程是修复骨缺损的一种有前途的方法，但固有的营养供应较差和大尺寸缺损的成骨速度慢是两个障碍。这导致迫切需要了解如何协调血管生成和骨生成以促进骨缺损愈合。本文在组织工程骨（TEB）中制备了聚L-赖氨酸包被的血管内皮生长因子/海藻酸盐控释微球（VEGF-AG-PLL），然后将该复合材料植入山羊股骨的CSBD中。动态髓内杆用于固定股骨并产生类似的生理轴向压缩。我们发现早期释放的VEGF不仅能促进血管生成，而且有利于成骨。同时，在早期阶段，生理轴向压缩可以调节血管生成并促进复合材料中VEGF的释放。后期，压缩可以加速TEB的重建。总之，本研究证明VEGF和压迫的协同应用可以加速TEB的重建；从而为未来临床修复大骨缺损提供新策略。
Angiogenesis and osteogenesis are essential for healing bone injuries, especially those related to critically sized bone defect (CSBD). Bone tissue engineering is a promising method for repairing bone defect, but the inherently poor nutrient supply and the slow osteogenesis of large size defect are two obstacles. This leads to an urgent need to understand how to orchestrate angiogenesis and osteogenesis for promoting bone defect healing. In this article, poly-L-lysine-coated vascular endothelial growth factor/alginate controlled releasing microspheres (VEGF-AG-PLL) were fabricated in tissue-engineered bone (TEB), and then the composite was implanted into the CSBD of goat femurs. Dynamic intramedullary rod was utilized to fixate the femur and produce an analogous physiological axial compression. We found that VEGF released in early stage not only promoted angiogenesis, but also brought benefit to osteogenesis. Meanwhile, in early stage physiological axial compression could regulate angiogenesis and facilitate VEGF release from the composite. In later stage, compression could accelerate reconstruction of TEB. In conclusion, this study demonstrated that the collaborative application of VEGF and compression could accelerate the reconstruction of TEB; thus, providing a new strategy for clinically repairing large bone defect in future.
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