Accelerated Bone Induction of Adult Rat Compact Bone Plate Scratched by Ultrasonic Scaler Using Acidic Electrolyzed Water.
Accelerated Bone Induction of Adult Rat Compact Bone Plate Scratched by Ultrasonic Scaler Using Acidic Electrolyzed Water.
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DOI：
10.3390/ma14123347
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发表时间：
2021-06-17
期刊：
影响因子：
--
通讯作者：
Upadhyaya C
中科院分区：
文献类型：
--
作者：
Shakya M;Murata M;Yokozeki K;Akazawa T;Nagayasu H;Adhikari BR;Upadhyaya C
新鲜致密骨是骨再生的候选移植材料，通常用于水平骨增强。然而，致密的钙化结构抑制生长因子的释放，限制细胞和血管的灌注。我们的目标是通过超声波治疗，以及使用市售的酸性电解水（AEW）进行部分脱矿，创造机械化学改变的致密颅骨。使用AEW （pH 2.3）或蒸馏水（DW, pH 5.6）作为冲洗剂，暴露11月龄Wistar大鼠顶骨，用压电超声清洗机尖端连续处理1min。取处理后的顶骨，切成5 × 5 × 1 mm3的钢板，移植到同基因大鼠背部皮下组织，分别于1、2、3周外植。AEW骨表现为不规则表面，深纳米微裂纹和脱钙区。SEM-EDS显示，与DW骨（0.86%）相比，AEW骨的残余钙含量较少（0.03%）。在动物实验中，AEW骨在2周诱导成骨。组织形态学分析显示，第2周和第3周AEW骨中新生骨面积明显增大。aew脱矿与超声治疗相结合的新技术将改善致密骨的表面积和三维结构，加速新骨的合成。
Fresh compact bone, the candidate graft material for bone regeneration, is usually grafted for horizontal bone augmentation. However, the dense calcified structure inhibits the release of growth factors and limits cellular and vascular perfusion. We aimed to create mechano-chemically altered dense skull bone by ultrasonic treatment, along with partial demineralization using commercially available acidic electrolyzed water (AEW). The parietal skull bone of an 11-month-old Wistar rat was exposed and continuously treated with a piezoelectric ultrasonic scaler tip for 1 min, using AEW (pH 2.3) or distilled water (DW, pH 5.6) as irrigants. Treated parietal bone was removed, cut into plates (5 × 5 × 1 mm3), grafted into the back subcutaneous tissues of syngeneic rats, and explanted at 1, 2, and 3 weeks. AEW bone showed an irregular surface, deep nano-microcracks, and decalcified areas. SEM-EDS revealed small amounts of residual calcium content in the AEW bone (0.03%) compared to the DW bone (0.86%). In the animal assay, the AEW bone induced bone at 2 weeks. Histomorphometric analysis showed that the area of new bone in the AEW bone at 2 and 3 weeks was significantly larger. This new combination technique of AEW-demineralization with ultrasonic treatment will improve the surface area and three-dimensional (3D) architecture of dense bone and accelerate new bone synthesis.
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影响因子：
--
作者：
Florencio-Silva R;Sasso GR;Sasso-Cerri E;Simões MJ;Cerri PS
通讯作者：
Cerri PS
影响因子：
3.7
作者：
Nakano Y;Akamatsu N;Mori T;Sano K;Satoh K;Nagayasu T;Miyoshi Y;Sugio T;Sakai H;Sakae E;Ichimiya K;Hamada M;Nakayama T;Fujita Y;Yanagihara K;Nishida N
通讯作者：
Nishida N
DOI：
10.1016/s0901-5027(98)80071-4
发表时间：
1998-10-01
影响因子：
2.4
作者：
Murata, M;Inoue, M;Nagai, N
通讯作者：
Nagai, N
影响因子：
3.9
作者：
Figueiredo, Margarida;Cunha, Sara;Figueiredo, Helena
通讯作者：
Figueiredo, Helena
影响因子：
6.2
作者：
Seref-Ferlengez, Zeynep;Basta-Pljakic, Jelena;Kennedy, Oran D.;Philemon, Claudy J.;Schaffler, Mitchell B.
通讯作者：
Schaffler, Mitchell B.