Quantotypic properties of QconCAT peptides targeting bovine host response to Streptococcus uberis.
Quantotypic properties of QconCAT peptides targeting bovine host response to Streptococcus uberis.
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DOI：
10.1021/pr201064g
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发表时间：
2012-03-02
影响因子：
4.4
通讯作者：
Bendixen, Emoke
中科院分区：
文献类型：
--
作者：
Bislev, Stine L.;Kusebauch, Ulrike;Codrea, Marius C.;Beynon, Robert J.;Harman, Victoria M.;Rontved, Christine M.;Aebersold, Ruedi;Moritz, Robert L.;Bendixen, Emoke
哺乳动物宿主对病原体的应答与体液中高丰度蛋白质的波动以及以相对低拷贝数表达的蛋白质（如从免疫细胞和内皮分泌的细胞因子）的调节相关。因此，有效监测与宿主对病原体的反应相关的蛋白质仍然是一项具有挑战性的任务。在本文中，我们提出了一个有针对性的蛋白质组分析的一组20种蛋白质，被广泛认为是关键球员和指标的牛宿主对乳腺炎病原体的反应。通过QconCAT方法获得来自这20种蛋白质中的每一种的两个一致蛋白质型肽的稳定同位素标记的变体。我们提出了这40个蛋白质型肽的定量特性，并讨论了它们在宿主病原体相互作用研究中的应用。我们的研究结果清楚地表明了对17种靶向宿主反应蛋白的稳健监测。其中12个在乳腺组织的简单提取中容易定量，而其余蛋白质的表达水平太低，无法直接和稳定定量;因此，其准确定量需要进一步分离乳腺组织。
Mammalian host response to pathogens is associated with fluctuations in high abundant proteins in body fluids as well as in regulation of proteins expressed in relatively low copy numbers like cytokines secreted from immune cells and endothelium. Hence, efficient monitoring of proteins associated with host response to pathogens remains a challenging task. In this paper we present a targeted proteome analysis of a panel of 20 proteins that are widely believed to be key players and indicators of bovine host response to mastitis pathogens. Stable isotope labeled variants of two concordant proteotypic peptides from each of these 20 proteins were obtained through the QconCAT method. We present the quantotypic properties of these 40 proteotypic peptides, and discuss their application to research in host pathogen interactions. Our results clearly demonstrate a robust monitoring of 17 targeted host-response proteins. Twelve of these were readily quantified in a simple extraction of mammary gland tissues, while the expression levels of the remaining proteins were too low for direct and stable quantification; hence their accurate quantification requires further fractionation of mammary gland tissues.
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影响因子：
9.9
作者：
Lange, Vinzenz;Picotti, Paola;Domon, Bruno;Aebersold, Ruedi
通讯作者：
Aebersold, Ruedi
影响因子：
2.6
作者：
Lutzow YC;Donaldson L;Gray CP;Vuocolo T;Pearson RD;Reverter A;Byrne KA;Sheehy PA;Windon R;Tellam RL
通讯作者：
Tellam RL
影响因子：
3.3
作者：
Bannerman, D. D.
通讯作者：
Bannerman, D. D.
影响因子：
4.4
作者：
Bannerman, DD;Paape, MJ;Hope, JC
通讯作者：
Hope, JC
DOI：
10.1128/cdli.11.3.463-472.2004
发表时间：
2004-05-01
期刊：
CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY
影响因子：
--
作者：
Bannerman, DD;Paape, MJ;Rainard, P
通讯作者：
Rainard, P