Clonal Evolution of Enterocytozoon bieneusi Populations in Swine and Genetic Differentiation in Subpopulations between Isolates from Swine and Humans.
Clonal Evolution of Enterocytozoon bieneusi Populations in Swine and Genetic Differentiation in Subpopulations between Isolates from Swine and Humans.
复制标题
DOI：
10.1371/journal.pntd.0004966
复制
发表时间：
2016-08
影响因子：
3.8
通讯作者：
Li W
中科院分区：
文献类型：
--
作者：
Wan Q;Xiao L;Zhang X;Li Y;Lu Y;Song M;Li W
比氏肠细胞虫是一种分布广泛的寄生虫，宿主间具有高度的遗传多样性。它的天然宿主仍然难以捉摸，关于种群结构的数据仅在灵长类动物的分离株中可用。本文报道了101 E.利用核糖体内转录间隔区（ITS）和四个小和微卫星标记的序列分析，从猪分离的bieneusi。强连锁不平衡（LD）和有限的遗传重组的存在下，表明克隆结构的人口。贝叶斯推断的遗传，结构分析，和主坐标分析分离的总人口为3个亚群（SP3至SP 5）的遗传分离的菌株在一定的地理水平。比较分析表明，SP3与SP 5在两个已知的E. bieneusi亚群（SP1和SP2）。人类E.在猪亚群SP 4中分离的bieneusi支持某些E. bieneusi分离物。网络分析表明，SP 5到SP3/SP 4和SP1到SP2的定向进化。高LD和推断的重组事件的低数目与SP2、SP3和SP 4中宿主适应的可能性一致。而SP1和SP 5的LD降低和遗传多样性高可能是寄主范围广和适应新寄主环境的结果。这些数据提供了证据表明，在某些E. bieneusi分离株属于人畜共患ITS组1。本研究对101个E.中国猪群中分离到的毕新氏菌。LD和重组事件的测量支持来自中国四个研究地区的菌株之间的克隆进化的发生。三个亚群（SP3至SP 5）与潜在的不同的主机范围内的分离株，这是遗传分化的两个现有的灵长类动物亚群SP1和SP2。种群遗传分析表明，SP1和SP 5中具有克隆结构的分离株可能是跨种传播的病原菌，具有人畜共患病的可能性，而SP2、SP3和SP 4中具有流行结构或宿主适应性的分离株可能在特定宿主中定殖。这些数据揭示了E. bieneusi在不同的主机。
Enterocytozoon bieneusi is a widespread parasite with high genetic diversity among hosts. Its natural reservoir remains elusive and data on population structure are available only in isolates from primates. Here we describe a population genetic study of 101 E. bieneusi isolates from pigs using sequence analysis of the ribosomal internal transcribed spacer (ITS) and four mini- and microsatellite markers. The presence of strong linkage disequilibrium (LD) and limited genetic recombination indicated a clonal structure for the population. Bayesian inference of phylogeny, structural analysis, and principal coordinates analysis separated the overall population into three subpopulations (SP3 to SP5) with genetic segregation of the isolates at some geographic level. Comparative analysis showed the differentiation of SP3 to SP5 from the two known E. bieneusi subpopulations (SP1 and SP2) from primates. The placement of a human E. bieneusi isolate in pig subpopulation SP4 supported the zoonotic potential of some E. bieneusi isolates. Network analysis showed directed evolution of SP5 to SP3/SP4 and SP1 to SP2. The high LD and low number of inferred recombination events are consistent with the possibility of host adaptation in SP2, SP3, and SP4. In contrast, the reduced LD and high genetic diversity in SP1 and SP5 might be results of broad host range and adaptation to new host environment. The data provide evidence of the potential occurrence of host adaptation in some of E. bieneusi isolates that belong to the zoonotic ITS Group 1. This study explored the genetic characteristics of the ITS and four mini- and microsatellite markers and assessed the population structure and substructures in 101 E. bieneusi isolates from pigs in China. The measures of LD and recombination events supported the occurrence of clonal evolution among the isolates from four study areas of China. Three subpopulations (SP3 to SP5) with potentially varied host ranges were identified within the isolates, which were genetically differentiated from two existing primate subpopulations SP1 and SP2. Population genetic analysis indicated that the isolates in SP1 and SP5 with clonal structure might be responsible for the cross-species transmission and thus have zoonotic potential, while the isolates in SP2, SP3, and SP4 with epidemic structure or host-adapted traits might colonize specific hosts. The data revealed the presence of clonality, potential host adaptation, and population differentiation of E. bieneusi in different hosts.
登录
查看更多内容
影响因子：
3.7
作者：
Maside X;Gómez-Moracho T;Jara L;Martín-Hernández R;De la Rúa P;Higes M;Bartolomé C
通讯作者：
Bartolomé C
影响因子：
3.7
作者：
Li W;Li Y;Li W;Yang J;Song M;Diao R;Jia H;Lu Y;Zheng J;Zhang X;Xiao L
通讯作者：
Xiao L
影响因子：
4
作者：
Li, Wei;Cama, Vitaliano;Xiao, Lihua
通讯作者：
Xiao, Lihua
黑龙江省猫犬隐孢子虫、比氏肠细胞虫和十二指肠贾第鞭毛虫的流行状况及遗传特征
影响因子：
2.6
作者：
Li, Wei;Li, Yijing;Xiao, Lihua
通讯作者：
Xiao, Lihua
中国非人灵长类比氏肠细胞虫的遗传多态性及人畜共患可能性
影响因子：
4.4
作者：
Karim, Md Robiul;Wang, Rongjun;Xiao, Lihua
通讯作者：
Xiao, Lihua