Evidence that part of a centromeric DNA region induces pseudohyphal growth in a dimorphic yeast, Candida maltosa
Evidence that part of a centromeric DNA region induces pseudohyphal growth in a dimorphic yeast, Candida maltosa
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着丝粒 DNA 区域的一部分诱导二态性酵母（麦芽糖假丝酵母）假菌丝生长的证据
DOI：
10.1128/jb.179.16.5030-5036.1997
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发表时间：
1997
影响因子：
3.2
通讯作者：
M. Takagi
中科院分区：
文献类型：
--
作者：
T. Nakazawa;T. Motoyama;H. Horiuchi;A. Ohta;M. Takagi
我们观察到，YCp型载体的着丝粒DNA（CEN）序列先前从基因组中分离，但不是一个YRP型载体缺乏CEN序列，诱导假菌丝生长的二型真菌，麦芽糖假丝酵母，这已被证明是密切相关的白色念珠菌的系统发育分析。CEN序列的缺失分析表明，完整的CEN序列是不需要的诱导，但它的一部分，具有部分着丝粒活性，是足够的诱导。通过筛选该酵母的基因文库中诱导假菌丝生长的序列，我们分离到三个不同的DNA片段，它们也具有部分的着丝粒样序列。这些片段的部分着丝粒活性通过三个标准来确认：携带这些片段的质粒的低拷贝数和高稳定性，以及用这些片段之一加上CEN序列的质粒DNA以高频率重排。此外，当通过定点诱变在CEN序列中突变通常在这四个序列的每一个中的一个拷贝中发现的GGTAGCG序列时，CEN序列的部分着丝粒活性和假菌丝生长诱导活性都丧失。这些结果表明，具有部分着丝粒活性的CEN区域可诱导假菌丝生长。麦芽糖。有人建议，一些细胞成分与着丝粒活性所需的序列包含GGTAGCG相互作用参与酵母形式和pseudohyphal形式的细胞之间的过渡的调节。
We observed that a YCp-type vector having the centromeric DNA (CEN) sequence previously isolated from the genome, but not a YRp-type vector lacking the CEN sequence, induced pseudohyphal growth in a dimorphic fungi, Candida maltosa, which had been shown to be closely related to Candida albicans by phylogenetic analysis. Deletion analysis of the CEN sequence revealed that the intact CEN sequence was not required for the induction, but part of it, having partial centromeric activity, was enough for the induction. By screening the gene library of this yeast for the sequences which induced pseudohyphal growth, we isolated three different DNA fragments which also had part of the centromere-like sequence. Partial centromeric activity of these fragments was confirmed by three criteria: low copy number and high stability of the plasmids carrying these fragments and rearrangement at high frequency of the plasmid DNA with one of these fragments plus the CEN sequence. Furthermore, when the GGTAGCG sequence commonly found in one copy in each of these four sequences was mutated in the CEN sequence by site-directed mutagenesis, both partial centromeric activity and pseudohyphal growth-inducing activity of the CEN sequence were lost. These results indicated that part of CEN region with partial centromeric activity induces pseudohyphal growth in C. maltosa. It is suggested that some cellular components which interact with the sequence containing GGTAGCG required for centromeric activity are involved in the regulation of the transition between yeast forms and pseudohyphal forms of the cells.
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影响因子：
3.3
作者：
KRON, SJ;STYLES, CA;FINK, GR
通讯作者：
FINK, GR
影响因子：
10.5
作者：
ROBERTS, RL;FINK, GR
通讯作者：
FINK, GR
DOI：
10.1073/pnas.93.23.13223
发表时间：
1996-11-12
影响因子：
11.1
作者：
Kohler, JR;Fink, GR
通讯作者：
Fink, GR
DOI：
10.1128/mmbr.54.3.226-241.1990
发表时间：
1990-09
期刊：
Microbiological reviews
影响因子：
--
作者：
Stewart;'. Scherer;A. P. T. Magee
通讯作者：
Stewart;'. Scherer;A. P. T. Magee
DOI：
10.1073/pnas.93.16.8419
发表时间：
1996-08-06
影响因子：
11.1
作者：
Lambrechts, MG;Bauer, FF;Pretorius, IS
通讯作者：
Pretorius, IS