Genome-edited TaTFL1-5 mutation decreases tiller and spikelet numbers in common wheat.
Genome-edited TaTFL1-5 mutation decreases tiller and spikelet numbers in common wheat.
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DOI：
10.3389/fpls.2023.1142779
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发表时间：
2023
影响因子：
5.6
通讯作者：
Gao, Xin-Qi
中科院分区：
文献类型：
--
作者：
Sun, Jing;Bie, Xiao Min;Chu, Xiao Li;Wang, Ning;Zhang, Xian Sheng;Gao, Xin-Qi
分蘖是小麦的一个重要农艺性状。它决定了枝条结构并影响谷物产量。TFL 1基因编码磷脂酰乙醇胺结合蛋白，参与植物发育中开花和芽结构的转变。然而，TFL 1同源物在小麦发育中的作用知之甚少。在本研究中使用CRISPR/Cas9介导的靶向诱变来产生一组具有单一、双重或三重无效tatfl 1 -5等位基因的小麦（菲尔德）突变体。小麦tatfl 1 -5突变体在营养生长期降低了单株分蘖数，在田间成熟期降低了单株有效分蘖数和每穗颖花数。RNA-seq分析表明，tatfl 1 -5突变体腋芽中生长素信号相关基因和细胞分裂素信号相关基因的表达发生了显著变化。结果表明，小麦TaTFL 1 -5s可能通过生长素和细胞分裂素信号途径参与分蘖调控。
Tillering is a critical agronomic trait of wheat (Triticum aestivum L.) that determines the shoot architecture and affects grain yield. TERMINAL FLOWER 1 (TFL1), encoding a phosphatidylethanolamine-binding protein, is implicated in the transition to flowering and shoot architecture in plant development. However, the roles of TFL1 homologs is little known in wheat development. CRISPR/Cas9-mediated targeted mutagenesis was used in this study to generate a set of wheat (Fielder) mutants with single, double or triple-null tatfl1-5 alleles. The wheat tatfl1-5 mutations decreased the tiller number per plant in the vegetative growth stage and the effective tiller number per plant and spikelet number per spike at maturity in the field. RNA-seq analysis showed that the expression of the auxin signaling–related and cytokinin signaling–related genes was significantly changed in the axillary buds of tatfl1-5 mutant seedlings. The results suggested that wheat TaTFL1-5s were implicated in tiller regulation by auxin and cytokinin signaling.
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影响因子：
5.3
作者：
CONLEY, TR;PARK, SC;SHIH, MC
通讯作者：
SHIH, MC
影响因子：
13.8
作者：
Gupta, Ajay;Hua, Lei;Zhang, Zhengzhi;Yang, Bing;Li, Wanlong
通讯作者：
Li, Wanlong
DOI：
10.1093/plcell/koab243
发表时间：
2021-12-03
期刊：
The Plant cell
影响因子：
--
作者：
Li K;Debernardi JM;Li C;Lin H;Zhang C;Jernstedt J;Korff MV;Zhong J;Dubcovsky J
通讯作者：
Dubcovsky J
影响因子：
11.6
作者：
Hanano, Shigeru;Goto, Koji
通讯作者：
Goto, Koji
影响因子：
7.4
作者：
Liu, Jie;Cheng, Xiliu;Sun, Jiaqiang
通讯作者：
Sun, Jiaqiang