Effect of DNA length on the nucleosome low salt transition.

Effect of DNA length on the nucleosome low salt transition.
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DNA长度对核小体低盐转变的影响。

DOI:
10.1093/nar/8.11.2475
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发表时间:
1980
影响因子:
14.9
通讯作者:
Cantor,CR
Cantor,CR
中科院分区:
生物学2区
文献类型:
--
作者:
Dieterich,AE;Eshaghpour,H;Crothers,DM;Cantor,CR

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用荧光标记的组蛋白研究了DNA长度对核小体低盐去折叠转变的影响。核小体是由平均长度为173和250个碱基对的大量DNA片段重构而形成的。这些核小体在以约7 mM离子强度为中心的转变中表现出构象变化,与标准145 bp核小体(1- 3 mM)观察到的构象变化非常不同。此外,173和250 bp核小体的构象变化涉及的离子是145 bp核小体的两倍。
The effect of DNA length on the low salt unfolding transition of nucleosomes has been studied by the use of fluorescently labeled histones. Nucleosomes were formed by the reconstitution of bulk DNA fragments averaging 173 and 250 base pairs in length. These nucleosomes exhibited a conformational change in a transition centered at about 7 mM ionic strength, very different from that observed for the standard 145 bp nucleosomes (1-3mM). In addition, the conformational change of the 173 and 250 bp nucleosomes involves twice as many ions as that of the 145 bp nucleosomes.
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